Development of a Laser-Based Technology for Rapid Point of Care Diagnosis of Blood-borne Infections

总结 本研究的目的是发展一种基于激光的分析方法， 即时血液诊断，用于检测被病毒、细菌和寄生虫感染的血液。等 仪器不需要高度专业化的培训， 一旦血液样本被引入进行分析，初步调查 与FDA生物制品评价和研究中心以及TriCore Reference合作 实验室表明，激光诱导击穿光谱（LIBS）结合 先进的数学和统计分析技术具有这种潜力。这件作品正 建议扩展这些调查，以更好地了解光谱信号 有助于区分单纯疱疹病毒、HIV、金黄色葡萄球菌， 人类血液中的克氏锥虫以及增强这些信号以建立可靠的 可部署在仪器上用于即时诊断的检测算法。

Summary The objective of this research is to develop a laser-based method of analysis into a point-of-care blood diagnostic to detect blood infected by viruses, bacteria, and parasites. Such an instrument will not require highly specialized training and will provide rapid identification of the contamination once a blood sample is introduced for analysis. Preliminary investigations in partnership with the FDA Center for Biologics Evaluation and Research and TriCore Reference Laboratories indicates that Laser-Induced Breakdown Spectroscopy (LIBS) combined with advanced mathematical and statistical analysis techniques has this potential. The work being proposed is to extend these investigations to better understand the spectroscopic signals contributing to the differentiation of Herpes Simplex Virus, HIV, Staphylococcus aureus, and Trypanosoma cruzi in human blood and means of enhancing these signals to build a reliable detection algorithm deployable on instrument for point-of-care diagnosis.