Development of a Laser-Based Technology for Rapid Point of Care Diagnosis of Blood-borne Infections

摘要 这项研究的目标是将一种基于激光的分析方法发展成一种 护理点血液诊断，以检测被病毒、细菌和寄生虫感染的血液。是这样的 仪器将不需要高度专业化的培训，并将提供快速识别 一旦血液样本被引入进行分析，就会受到污染。年的初步调查 与FDA生物制品评估和研究中心和Tricore参考中心建立合作伙伴关系 实验室表明，激光诱导击穿光谱(LIBS)与 先进的数学和统计分析技术具有这种潜力。这项工作是 建议扩大这些研究，以更好地理解光谱信号 有助于区分单纯疱疹病毒、艾滋病毒、金黄色葡萄球菌和 人血中克氏锥虫和增强这些信号的手段建立可靠 检测算法可部署在仪器上进行护理点诊断。

Summary The objective of this research is to develop a laser-based method of analysis into a point-of-care blood diagnostic to detect blood infected by viruses, bacteria, and parasites. Such an instrument will not require highly specialized training and will provide rapid identification of the contamination once a blood sample is introduced for analysis. Preliminary investigations in partnership with the FDA Center for Biologics Evaluation and Research and TriCore Reference Laboratories indicates that Laser-Induced Breakdown Spectroscopy (LIBS) combined with advanced mathematical and statistical analysis techniques has this potential. The work being proposed is to extend these investigations to better understand the spectroscopic signals contributing to the differentiation of Herpes Simplex Virus, HIV, Staphylococcus aureus, and Trypanosoma cruzi in human blood and means of enhancing these signals to build a reliable detection algorithm deployable on instrument for point-of-care diagnosis.