权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Conserved RNA Secondary Structures in three Betacoronaviruses: MHV, BCoV, and MERS-CoV

三种 β 冠状病毒的保守 RNA 二级结构：MHV、BCoV 和 MERS-CoV

基本信息

批准号：
9016635
负责人：
JULIAN L LEIBOWITZ
金额：
$ 19.94万
依托单位：
TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9016635
关键词：
3&apos Untranslated Regions 5&apos Untranslated Regions Animals Antiviral Therapy Biochemical Biological Models Cattle China Chiroptera Cis-Acting Sequence Coronavirus Coronavirus Infections Coupled Data Disease Outbreaks Domestic Animals Elements Enteral Epidemic Event Family Future Genetic Transcription Genome Goals Growth Higher Order Chromatin Structure Human Indium Length Liver diseases Lower Respiratory Tract Infection Lung diseases Methods Middle East Respiratory Syndrome Coronavirus Modeling Murine hepatitis virus Nidovirales Nucleotides Play Primer Extension RNA RNA Sequences RNA chemical synthesis Regulation Resolution Role SARS coronavirus Severe Acute Respiratory Syndrome Signal Transduction Structure Testing Upper Respiratory Infections Viral Genome Virus Virus Replication Wild Animals cis acting element genetic approach genomic RNA insight man member mortality nervous system disorder new therapeutic target next generation sequencing novel pathogen prevent public health relevance research study respiratory reverse genetics small molecule

项目摘要

DESCRIPTION (provided by applicant): The Betacoronavirus genus includes two viruses, SARS-CoV and MERS-CoV that emerged from animal reservoirs to produce severe pulmonary disease in man in 2002, and 2012, respectively. In this application we propose to study the RNA secondary structure of MERS-CoV and two related coronaviruses, mouse hepatitis virus (MHV) and bovine coronavirus (BCoV) with the goal of identifying and functionally characterizing novel conserved RNA secondary structures in the genomes of these viruses. In aim one we will employ a state-of- the-art, high throughput approach, SHAPE-MaP, to generate biochemical data that allows the creation of highly accurate models of RNA secondary structures of large RNAs, such as coronavirus genomes. Through comparison of the RNA secondary structures of the genomes of MHV, BCoV, and MERS-CoV we will seek previously undiscovered phylogenetically conserved RNA secondary structures that are strong candidates to play a functional role in viral replication. In aim 2 we will employ reverse genetic approaches to characterize the functional role in MHV and MERS-CoV replication of conserved secondary structures identified in aim 1.

描述（由申请人提供）：β冠状病毒属包括两种病毒，SARS-CoV和MERS-CoV，分别于2002年和2012年从动物宿主中出现，在人类中产生严重的肺部疾病。在本申请中，我们建议研究MERS冠状病毒和两种相关冠状病毒，小鼠肝炎病毒（MHV）和牛冠状病毒（BCoV）的RNA二级结构，目的是鉴定和功能表征这些病毒基因组中的新型保守RNA二级结构。在目标一中，我们将采用最先进的高通量方法SHAPE-MaP来生成生化数据，从而可以创建大RNA（如冠状病毒基因组）的RNA二级结构的高度准确模型。通过比较MHV，BCoV和MERS-CoV基因组的RNA二级结构，我们将寻找以前未发现的在病毒复制中发挥功能作用的强有力的候选人的遗传保守的RNA二级结构。在目标2中，我们将采用反向遗传方法来表征目标1中鉴定的保守二级结构在MHV和MERS-CoV复制中的功能作用。