Protein Production and Protein Interaction Core

丝状病毒是疾控中心A类优先病原体，可以颠覆宿主免疫系统，促进病毒 复制，导致人间病例死亡率高达90%。埃博拉病毒(EBOV)依赖RNA的RNA 聚合酶(RDRP)复合体是复制的关键。然而，调节病毒RNA合成的机制 或者，改变病毒复制的因素还不清楚。蛋白质生产与蛋白质相互作用 核心(核心B)将为整个科学任务提供关键试剂和支持，并促进 病毒-病毒和病毒-宿主相互作用的特征。具体地说，Core B将提供一组独特的 将在项目和核心中传播的标准化试剂和验证数据。核心B 确定了实验的优先顺序，以确保试剂随时可供项目使用，并 核心。核心B将产生毫克量的高纯度重组病毒RDRP蛋白，这将是 用于评价菌株的相似性/差异性，以及用于单抗的研制和合成 抗体的产生。核心B还将产生由项目1和3确定的重组新宿主因子 将用于验证和表征EBOV RDRP复合体和新宿主因子之间的接口。

Filoviruses are CDC category A priority pathogens that can subvert host immune systems to facilitate viral replication, resulting in human case fatality rates as high as 90%. The Ebola virus (EBOV) RNA-dependent RNA polymerase (RDRP) complex is critical for replication. However, the mechanisms regulating viral RNA synthesis or the factors that modify viral replication are not understood. The Protein Production and Protein Interaction Core (Core B) will provide critical reagents and support for the overall scientific mission and facilitate the characterization of viral-viral and viral-host interactions. Specifically, Core B will provide a unique set of standardized reagents and validation data that will be disseminated amongst the Projects and Cores. Core B has prioritized experiments in order to ensure that reagents will be readily available for use by the Projects and Cores. Core B will generate milligram quantities of highly purified recombinant viral RDRP proteins that will be used for evaluation of strain similarities/differences and for both monoclonal antibody development and synthetic antibody generation. Core B will also produce recombinant novel host factors identified by Projects 1 and 3 that will be used to validate and characterize interfaces between the EBOV RDRP complex and novel host factors.