Protein Production and Protein Interaction Core

丝状病毒是CDC A类优先病原体，其可以破坏宿主免疫系统以促进病毒感染。 复制，导致人类病例死亡率高达90%。埃博拉病毒（EBOV）RNA依赖性RNA 聚合酶（RDRP）复合物对于复制至关重要。然而，调节病毒RNA合成的机制 或者改变病毒复制的因素还不清楚。蛋白质的产生与相互作用 核心（核心B）将为整个科学使命提供关键试剂和支持，并促进 病毒-病毒和病毒-宿主相互作用的表征。具体而言，核心B将提供一组独特的 标准化试剂和验证数据将在项目和核心之间传播。核心B 已对实验进行了优先排序，以确保试剂可随时供项目使用， 丹核心B将产生毫克量的高度纯化的重组病毒RDRP蛋白， 用于评价菌株相似性/差异以及单克隆抗体开发和合成 抗体生成。核心B还将产生由项目1和3鉴定的重组新宿主因子， 将用于验证和表征EBOV RDRP复合物和新宿主因子之间的界面。

Filoviruses are CDC category A priority pathogens that can subvert host immune systems to facilitate viral replication, resulting in human case fatality rates as high as 90%. The Ebola virus (EBOV) RNA-dependent RNA polymerase (RDRP) complex is critical for replication. However, the mechanisms regulating viral RNA synthesis or the factors that modify viral replication are not understood. The Protein Production and Protein Interaction Core (Core B) will provide critical reagents and support for the overall scientific mission and facilitate the characterization of viral-viral and viral-host interactions. Specifically, Core B will provide a unique set of standardized reagents and validation data that will be disseminated amongst the Projects and Cores. Core B has prioritized experiments in order to ensure that reagents will be readily available for use by the Projects and Cores. Core B will generate milligram quantities of highly purified recombinant viral RDRP proteins that will be used for evaluation of strain similarities/differences and for both monoclonal antibody development and synthetic antibody generation. Core B will also produce recombinant novel host factors identified by Projects 1 and 3 that will be used to validate and characterize interfaces between the EBOV RDRP complex and novel host factors.