Chlamydia-induced host protein degradation and its impact on the adaptive immune response.

 描述(申请人提供)专性细胞内细菌沙眼衣原体在人类和动物中都会引起严重的疾病。了解其与宿主细胞相互作用的机制对于控制感染至关重要。沙眼衣原体可以改变其感染细胞内宿主蛋白的稳定性，其对宿主和细菌的影响尚不完全清楚。我们已经确定，感染不依赖于mRNA转录水平而破坏和降解模型宿主蛋白，结果是增加了来自宿主蛋白的多肽水平。这些多肽可以与MHC I类分子结合，并在细胞表面呈递给CD8+T细胞。我们将通过确定哪些衣原体基因对这种表型负责，来确定沙眼衣原体如何改变宿主蛋白的稳定性。我们还将确定增强宿主多肽呈递是否会降低宿主细胞呈递衣原体抗原多肽的效率。

 DESCRIPTION (provided by applicant) The obligate intracellular bacteria Chlamydia trachomatis causes serious diseases in both humans and animals. Understanding the mechanisms of its interactions with host cells is essential for controlling infections. C. trachomatis can alter the stability of intracellular host proteins in the cells it infects and the consequences for the host and bacterium are not fully understood. We have determined that infection destabilizes and degrades a model host protein independently of mRNA transcript levels and as a result increases the levels of peptides derived from the host protein. These peptides can bind to MHC class I molecules and be presented to CD8+ T cells at the cell surface. We will determine how C. trachomatis alters the host protein stability by determining which chlamydial genes are responsible for this phenotype. We will also determine if enhancing host peptide presentation decreases the efficiency of the host cell to present peptides derived from chlamydial antigens.