The histone demethylase KDM3A upregulates MCAM to promote Ewing Sarcoma metastasis
The histone demethylase KDM3A upregulates MCAM to promote Ewing Sarcoma metastasis
批准号:
9050504
负责人:
Marybeth Sechler Lupo
金额:
$3.28万
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-03-02 至 2019-03-01
关键词:
AnoikisBone TissueCD146 antigenCell Adhesion MoleculesCell LineCellsChildChildhood Solid NeoplasmDataDependenceDiseaseDisease ProgressionDisease remissionDistalDistantDropsEndothelial CellsEpigenetic ProcessEwings sarcomaGenesGoalsGrowthIn VitroInjection of therapeutic agentLaboratoriesLeadLifeLocalized DiseaseMalignant NeoplasmsMediator of activation proteinMicrometastasisModelingNeoplasm MetastasisOutcomePTK2 genePathogenesisPathway interactionsPatientsPediatric NeoplasmPhenotypePre-Clinical ModelPrimary NeoplasmProteinsPublishingRecurrenceRegulationRegulatory PathwayRelapseResistanceRoleSignal TransductionSiteStagingSurvival RateTailTestingTumor Cell MigrationUp-RegulationVeinsWorkcancer typecell motilitychemotherapyhigh riskhistone demethylaseimprovedin vitro Assayin vivoknock-downmigrationnew therapeutic targetnovel therapeutic interventionnovel therapeuticsoverexpressionpediatric patientspreventpromoterpublic health relevanceresearch studysarcomasoft tissuetargeted treatmenttherapeutic targettumor
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英文摘要
DESCRIPTION (provided by applicant): The long term goal of this proposal is to identify possible new therapeutic targets for Ewing Sarcoma. Once a patient develops metastasis to distant sites, his/her 5-year survival drops to 30%. Moreover, of the 70% of patients treated with initially localized disease, 30% will relapse at metastatic sites within a few years of initial remission. As Ewing Sarcoma is such an aggressive disease with strong propensity for early spread, new therapies that could potentially prevent tumor spread are in particular need. Our laboratory has identified an epigenetic modifier, KDM3A, as a positive regulator of Ewing Sarcoma migration and in vivo metastasis. Further analysis has shown that KDM3A holds regulatory power over a number of genes which promote metastasis, including Melanoma Cell Adhesion Molecule (MCAM). MCAM is overexpressed in several other types of cancers, and we have shown it to promote tumor cell migration. Importantly, MCAM has recently been identified as overexpressed in many different kinds of pediatric tumors, including Ewing Sarcoma. Our own preliminary data in conjunction with published work from other models lead to our hypothesis that KDM3A promotes Ewing sarcoma growth and dissemination through upregulation of MCAM. The following specific aims will test this hypothesis. Specific aim 1. Determine the role and mechanism of MCAM in KDM3A- induced metastatic phenotypes in Ewing Sarcoma in vitro. Specific aim 2. Determine the relative effects of KDM3A, MCAM and FAK inhibition on Ewing sarcoma metastasis in vivo. Understanding how KDM3A and its downstream target MCAM function to promote metastasis, may help us identify new therapeutic approaches for Ewing Sarcoma.
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