Science Core B

项目摘要/摘要 我们广泛的长期目标是从一个集中的科学核心提供试剂、模型和专业知识 向本计划的所有参与者提供便利。核心运营代表着提供服务和服务之间的平衡 以既定程序为基础，并在方法和协议方面不断创新。安全是一个特殊的问题 核心指导原则和信息系统是由核心参与者在 TSE的研究，安全均质和其他生物危害操作程序的设施，Pron处理和 去污。协议标准化是另一个重要目标，这样方案的所有方面都有 获得使用一致的方法生产和操作的试剂和服务 消息来源。具体目标：目的I-提供纯化的重组PrP，用于定量PrP的分析，并用于 PrP转化的分析；目的II-为纯化的抗PrP单抗提供良好的 已定义的特性；目的III-提供特性化的转基因和基因靶向小鼠模型；目的IV-至 使用灵敏的、高通量的基于细胞的分析来量化普恩；目标V--提供各种服务 分析普恩病毒感染的病理后果。科学的核心驻留在卓越 普恩病毒疾病研究的环境。个别项目内的研究将以接近尾声进行。 与Science Core合作，利用标准化的模型和专业知识 和一致的方法，以及统一一致的高质量试剂。核心的活动是 对于成功实现计划目标至关重要。

Project Summary / Abstract Our broad, long-term objectives are to supply reagents, models and expertise from a centralized Science Core facility to all participants of the Program. Core operation represents a balance between providing services based on established procedures, and continued innovation in methods and protocols. Safety is a particular guiding principle of the Core, and is are addressed by the many years of experience of Core participants in the study of TSEs, facilities for safe homogenization and other biohazard operating procedures, prion handling and decontamination. Protocol standardization is another crucial goal, so that all aspects of the Program have access to reagents and services produced and operated using consistent methods from a constant, reliable source. Specific Aims: Aim I - To supply purified recombinant PrP for assays to quantify prions, and for analyses of PrP conversion; Aim II - To supply purified anti-PrP monoclonal antibodies (mAbs) with well- defined properties; Aim III - To supply characterized transgenic and gene targeted mouse models; Aim IV - To quantify prions using sensitive, high throughput cell-based assays; and Aim V - To provide a variety of services to analyze pathological consequences of prion infection. The Science Core resides in outstanding environments for studies of prion diseases. Studies within individual Projects will be conducted in close collaboration with the Science Core to take advantage of the availability of models and expertise, standardized and consistent methodologies, and uniformly consistent, high quality reagents. The activity of the Core is essential for the successful accomplishment of Program goals.