Human Embryonic Stem Cell Core laboratory

核心A提供成功完成本计划内每个项目所必需的专业知识和资源。核心A被安置在埃里森干细胞核心中心(ESCC)，这是一个专门设计和建造的设施，提供组织培养罩、大量蔡司显微镜、活细胞成像能力和耐缺氧孵化器。此外，Core A提供了用于培养和维护人类多能干细胞的经过验证的试剂，最大限度地减少了不同供应商或批次之间可能发生的实验差异。核心A还教授和维护一个“最佳实践”环境，将可能导致污染和文化漂移的偏差降至最低。然而，也许是最伟大的 重要的是在ESCC内POI调查员的共同本地化所产生的优势。通过汇集来自不同学科的研究人员，ESCC汇集了专业知识。这使得来自一个实验室的研究人员可以立即受益于其他实验室取得的进展，有助于识别多潜能系之间的重要生物学差异，并促进受益于多个群体贡献的合作努力；最近的一个例子是采用无载体方法来诱导多潜能干细胞(IPSC)的产生。 核心A将通过提供核心服务、将其知识和资源提供给POI调查员以及发展新的知识和能力，促进整体POI的目标。在目前的资助期内，Core A为进一步加深我们对干细胞生物学的知识做出了重要贡献。目前的应用建议继续这一思路，通过鉴定新一代“幼稚的”人类多能干细胞系，开发将提高人类多能干细胞系产生感兴趣组织的可预测性的培养条件，并通过寻求确定多能性和定向分化的代谢驱动因素。

Core A provides expertise and resources that are essential for successful completion of each of the Projects within this Program. Core A is housed in the Ellison Stem Cell Core (ESCC), a purpose-designed and built facility providing access to tissue culture hoods, a number of Zeiss microscopes, live cell imaging capability, and hypoxia-ready incubators. Furthermore, Core A provides access to validated reagents for the culture and maintenance of human pluripotent stem cells, minimizing experimental variability that can occur between different vendors or batches. Core A also teaches and maintains a "best practices" environment that minimizes deviations that can lead to contamination and culture drift. However, perhaps of greatest importance are the advantages that result from the co-localization of POI investigators within the ESCC. By aggregating researchers from different disciplines, the ESCC aggregates expertise. This allows researchers from one laboratory to immediately benefit from advances made by other laboratories, facilitates the identification of important biological differences between pluripotent lines, and fosters collaborative efforts that benefit from contributions from multiple groups; a recent example being the adoption of vector-free methods for induced pluripotent stem cell (iPSC) generation. Core A will facilitate the goals of the overall POI through the provision of core services, by placing its knowledge and resources at the disposal of POI investigators, and by developing new knowledge and capabilities. During the current funding period Core A has made important contributions toward furthering our knowledge of stem cell biology. The current application proposes to continue in this vein by characterizing a new generation of "naive-like" human pluripotent stem cell lines, by developing culture conditions that will enhance the predictability with which human pluripotent stem cell lines generate tissues of interest, and by seeking to identify metabolic drivers of pluripotency and directed differentiation.