Sensor arrays based on molecularly imprinted polymers for diagnosis of Sjogren's syndrome

基于分子印迹聚合物的传感器阵列用于诊断干燥综合征

• 批准号: 9245475
• 负责人: Eric V. Anslyn
• 金额: $ 34万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-23 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9245475
• 关键词: Affect; Affinity; Antibodies; Autoimmune Diseases; Behavior; Beryllium; Binding Sites; Biological Markers; Biopsy; Biosensor; Clinical; Collection; Deglutition; Detection; Diagnosis; Diagnostic; Diffusion; Disease; Early Diagnosis; Electrostatics; Elements; Enzymes; Formulation; Gland; Goals; Gold; Inflammation; Ionic Strengths; Label; Lacrimal gland structure; Liquid substance; Lymphocyte; Metals; Methods; Molecular; Monitor; Nanotechnology; Optics; Oral cavity; Patients; Pattern; Phase; Polymers; Property; Protein Engineering; Proteins; Quality of life; Radioactive; Refractive Indices; Saliva; Salivary; Salivary Glands; Signal Transduction; Silicon Dioxide; Sjogren' s Syndrome; Solid; Staging; Surface; Surface Plasmon Resonance; Swelling; Testing; Work; associated symptom; base; biochemical tools; cost; covalent bond; cross reactivity; crosslink; design; imprint; improved; macromolecule; molecular recognition; monomer; nanomaterials; nanoparticle; nanoshell; optical sensor; protein biomarkers; receptor; research study; sensor

PROJECT SUMMARY Diagnosing Sjögren's syndrome (SS) in the early stages of the disease can help mitigate damage from systemic manifestations that occur later in the disease. Current tests for establishing an SS diagnosis include salivary gland biopsy to look for lymphocytic inflammation and using radioactive labeling to test salivary gland function. While these tests are effective in the diagnosis of SS, the multiplex biosensor developed in this proposal will provide a low-cost method for detecting molecular evidence of both salivary/lacrimal gland hypofunction and lymphocytic inflammation without the need for invasive testing. Molecularly imprinted polymers (MIPs) are synthetic materials capable of semi-selective molecular recognition and are lower cost and more environmentally robust than antibodies. In this proposal, MIPs will be synthesized on the surface of nanomaterials with unique optical properties for use in sensing applications. Specifically, collections of MIP- coated gold nanomaterials will be employed in “differential sensing,” routines to monitor biomarkers of lymphocytic inflammation and gland hypofunction simultaneously, providing a more comprehensive view of protein mixtures. Instead of identifying a single biomarker, the developed sensor arrays will generate a pattern based on numerous biomarkers, providing a more robust diagnostic tool for biochemical changes. The overall hypothesis of this proposal is that imprinted nanomaterials in optical sensor arrays may detect biomarkers that could be used to establish an SS diagnosis in symptomatic patients. The specific goals of this proposal are (Aim 1) to rationally choose strong-bonding monomers (non-covalent or reversible-covalent) to improve MIP affinity, (Aim 2) to synthesize MIPs on the surface of gold nanomateirals to generate receptors with built-in signal transduction and (Aim 3) to test these imprinted nanomaterials in sensor arrays to detect SS biomarkers.

项目总结 在疾病的早期阶段诊断干燥综合征(SS)有助于减轻 在疾病后期出现的全身性表现。目前用于确定SS诊断的测试包括 唾液腺活检以寻找淋巴细胞性炎症，并使用放射性标记检测唾液腺 功能。虽然这些测试在SS的诊断中是有效的，但在此基础上开发的复合生物传感器 该提案将提供一种低成本的方法来检测唾液/泪腺的分子证据。 功能减退和淋巴细胞炎，不需要侵入性测试。分子印迹 聚合物(MIP)是一种具有半选择性分子识别能力且成本较低的合成材料 而且比抗体更环保。在这项提议中，分子印迹聚合物将在表面合成 具有独特光学性能的纳米材料，用于传感应用。具体地说，MIP集合- 包覆的金纳米材料将被用于“差分传感”，常规的监测生物标记物 淋巴细胞炎和腺体功能减退的同时，提供了更全面的观点 蛋白质混合物。开发的传感器阵列将产生一种模式，而不是识别单个生物标志物 基于众多生物标志物，为生化变化提供更强大的诊断工具。整体而言 这一提议的假设是，光学传感器阵列中的印迹纳米材料可以检测到 可用于在有症状的患者中建立SS诊断。这项提议的具体目标是 (目标1)合理选择强键单体(非共价或可逆共价)，以提高MIP 亲和力，(目标2)在金纳米材料表面合成分子印迹聚合物，以产生内置受体 目的3)在传感器阵列中测试这些印迹纳米材料，以检测SS生物标记物。