ShEEP Request for Zeiss LSM880 Confocal Microscope

通过这一申请，我们要求ORD共享设备评估计划（SheEEP）提供资金， 购买一台新的共聚焦显微镜，安置在爱荷华州市的炎症项目实验室 VA医疗中心具体地，我们寻求获得由美国加利福尼亚州的加利福尼亚州制造的型号LSM 880共聚焦显微镜。 卡尔蔡司公司这次收购的理由有三个。首先，我们目前的共聚焦显微镜，一台蔡司 LSM 510已经有18年的历史了，从2016年9月起将被正式淘汰。因此，零件和 维护将不再可用，在许多研究中起关键作用的仪器 程序将无法使用。第二，我们有机会利用20%的以旧换新 购买LSM 880的折扣。第三，也是最重要的一点，LSM 880有几个特点 使其处于共聚焦技术的前沿。因此，获取LSM 880将支持切割边缘 研究，并将允许开发新的研究项目和科学家之间的新合作， 爱荷华州市退伍军人事务部研究项目，该项目长期以来一直保持卓越和成就。 我们的研究组合是多样化的，包括几种传染病，炎症和 免疫，青光眼和失明，心脏病，以及焦虑，抑郁，创伤后应激障碍， 老年痴呆症，帕金森氏症和其他神经系统疾病。共聚焦显微镜很重要 所有这些研究，因为它允许基本的细胞过程在高放大率下可视化， 免疫系统、大脑、心脏和其他组织的固定细胞。具体而言，研究小组和项目， 其中LSM 880是关键的：1）操纵巨噬细胞中的凋亡、焦亡和自噬， 土拉热弗朗西斯菌（艾伦博士）。2)诱导中性粒细胞亚型分化和DNA释放， 幽门螺杆菌感染（艾伦博士）。3)微囊泡（外泌体和外泌体）释放的研究 细菌感染（艾伦博士和恶心）。4)青光眼期间视网膜神经节细胞死亡的研究（Dr. 安德森）。5)Podosomes在青光眼和癌细胞运动中的作用（安德森博士）。6)研究 青光眼中的轴突线粒体动力学（安德森博士）。7)金黄色葡萄球菌活生物膜的研究 (Dr. Horswill）。8)中性粒细胞运输至皮肤中寄生虫感染部位的分析， 婴儿利什曼原虫感染期间巨噬细胞内的寄生虫外泌体（Wilson博士）。9)研究 各种感染性和炎症性疾病患者的中性粒细胞可塑性（Wilson和艾伦博士）。10）、 胶质细胞成熟因子在脑内的丰度和分布与脑胶质瘤发病机制的研究 阿尔茨海默氏症和帕金森氏症，以及治疗干预的机会（扎希尔博士）。11)分析 嗜中性粒细胞颗粒和吞噬体中含有S.金黄色葡萄球菌（恶心博士）。12)B细胞TRAF3的研究， FRET（Bishop博士）。13)定位正常和突变的酸敏感离子通道在大脑中，以阐明 它们在突触可塑性、焦虑症和创伤后应激障碍方面的功能（Wemmie博士）。 因此，LSM 880的获取将不仅仅在爱荷华州城市VA维持共焦显微镜。相反， 尖端技术将有助于确保我们在各自领域保持领先地位， 研究改善退伍军人的健康。

With this application we request funds from the ORD Shared Equipment Evaluation Program (SheEEP) to purchase a new confocal microscope to be housed in the Inflammation Program laboratories of the Iowa City VA Medical Center. Specifically, we seek to obtain a model LSM 880 confocal microscope manufactured by Carl Zeiss, Inc. The rationale for this purchase is threefold. First, our current confocal microscope, a Zeiss LSM 510, is 18 years old, and as of September 2016 will be officially obsolete. As such, parts and maintenance will no longer be available, and the instrument, which plays a critical role in many research programs, will become unusable. Second, we have the opportunity to take advantage of a 20% trade-up discount on purchase of the LSM 880. Third and most importantly, there are several features of the LSM 880 that place it at the forefront of confocal technology. Thus, acquisition of an LSM 880 will support cutting edge research and will allow the development of new research projects and new collaborations among scientists of the Iowa City VA research program, which has a long-standing record of excellence and achievement. Our research portfolio is diverse and includes studies of several infectious diseases, inflammation and immunity, glaucoma and blindness, and heart disease, as well as studies of anxiety, depression, PTSD, Alzheimer's disease, Parkinson's disease and other neurological disorders. Confocal microscopy is important to all these studies as it allows fundamental cellular processes to be visualized at high magnification in live and fixed cells of the immune system, brain, heart and other tissues. Specifically, research groups and projects for which the LSM 880 is critical are: 1) Manipulation of apoptosis, pyroptosis and autophagy in macrophages by Francisella tularensis (Dr. Allen). 2) Induction of neutrophil subtype differentiation and DNA release upon Helicobacter pylori infection (Dr. Allen). 3) Studies of microvesicle (ectosome and exosome) release during bacterial infection (Drs. Allen and Nauseef). 4) Studies of retinal ganglion cell death during glaucoma (Dr. Anderson). 5) The role of podosomes in glaucoma and cancer cell motility (Dr. Anderson). 6) Studies of axonal mitochondrial dynamics in glaucoma (Dr. Anderson). 7) Studies of live Staphylococcus aureus biofilms (Dr. Horswill). 8) Analysis of neutrophil trafficking to sites of parasite infection in the skin, and release of parasite exosomes inside macrophages during infection with Leishmania infantum (Dr. Wilson). 9) Studies of neutrophil plasticity in patients with various infectious and inflammatory disorders (Drs. Wilson and Allen). 10) Studies of Glial Maturation Factor abundance and distribution in the brain with respect to pathogenesis of Alzheimer's and Parkinson's disease, and opportunities for therapeutic intervention (Dr. Zaheer). 11) Analyses of neutrophil granules and phagosomes containing S. aureus (Dr. Nauseef). 12) Studies of B-cell TRAF3 using FRET (Dr. Bishop). 13) Localization of normal and mutated acid-sensing ion channels in the brain to elucidate their functions with respect to synaptic plasticity, anxiety disorders, and PTSD (Dr. Wemmie). Thus, acquisition of an LSM 880 will not merely maintain confocal microscopy at the Iowa City VA. Rather, this cutting edge technology will help to ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran's health.