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Topical application of heterologous protein-expressing Staphylococcus epidermidis for potential therapeutic treatment of skin diseases

Topical application of heterologous protein-expressing Staphylococcus epidermidis for potential therapeutic treatment of skin diseases
表达异源蛋白的表皮葡萄球菌的局部应用对皮肤病的潜在治疗作用
批准号:
9202769
负责人:
LEONARD M MILSTONE
金额:
$15.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-08-15 至 2017-07-31

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中文摘要
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英文摘要
PROJECT SUMMARY Current treatment options for a number of skin diseases generally aim for symptomatic relief and fail to address underlying pathophysiological changes leading to skin disease. In the case of genetic mutations that cause loss of function mutations, supplementation of the missing protein is a viable approach to treatment. However, delivery of functional protein to the target keratinocytes presents a significant challenge; moreover, the cost of protein production and purification creates a significant hurdle to developing a commercially viable treatment. Finally, given the natural turnover rate in the skin, constant reapplication of protein would be needed, which compounds issues related to cost of treatment, adherence, and convenience. Azitra is a preclinical company focused on microbiome-based therapeutics. We are developing a platform that consists of an engineered S. epidermidis that is able to establish residence on human skin and secrete therapeutic protein in situ. An ointment with an inoculum of such bacteria could be infrequently applied to skin, providing constant, low-cost, convenient delivery of therapeutic protein. Natural properties of S. epidermidis also include secretion of antimicrobial peptides against S. aureus and stimulation of regulatory T-cells, making S. epidermidis an ideal secretion platform. The proposed Phase I research plan will focus on proof-of-concept studies to determine the spatio-temporal growth characteristics of our S. epidermidis production and delivery system (Aim 1) and to characterize the spatio-temporal dynamics of sGFP that secreted by S. epidermidis in a skin equivalent model (Aim 2). In the next phase (Phase II) following this STTR Phase I project. The application of this platform will be tested with filaggrin secreted by S. epidermidis in pre- clinical models of atopic dermatitis.
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Frontiers in Ichthyosis Research
DERMATOREMEDIATION OF IRON OVERLOAD
  • 批准号:
    6621151
  • 项目类别:
  • 资助金额:
    $19.79万
  • 财政年份:
    2002
  • 负责人:
    LEONARD M MILSTONE
  • 依托单位:
DERMATOREMEDIATION OF IRON OVERLOAD
  • 批准号:
    7118485
  • 项目类别:
  • 资助金额:
    $4.09万
  • 财政年份:
    2002
  • 负责人:
    LEONARD M MILSTONE
  • 依托单位:
Dermatoremediation of Iron Overload
  • 批准号:
    7896497
  • 项目类别:
  • 资助金额:
    $30.87万
  • 财政年份:
    2002
  • 负责人:
    LEONARD M MILSTONE
  • 依托单位:
海外基金