TR&D 1 Isobaric Mass Tags for Ultra-Plexed Protein Quantification p. 398

TR

• 批准号: 8998784
• 负责人: LINGJUN LI
• 金额: $ 36.83万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-05 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8998784
• 关键词: Benchmarking; Biological; Biology; Chemicals; Collection; Complex; Data; Development; Ensure; Evaluation; Generations; Label; Leucine; Liquid substance; Medicine; Methods; Neutrons; Peptides; Performance; Preparation; Proteins; Proteome; Proteomics; Sampling; Scanning; Science; System; Technology; Time; Tissues; Work; base; cost effective; data acquisition; instrument; member; new technology; novel; parallel processing; protein aminoacid sequence; research study; translational medicine

Project Summary The continued development of protein quantification technologies is key to modern biology and medicine. Indeed, the ability to parallel process samples during quantitative analysis (multiplex) is essential for many of the proposed DBP applications. Sample multiplexing via chemical labeling offers numerous key advantages. First, parallel processing of samples in a single mass spectrometric analysis reduces the amount of material required from any one sample; this is particularly relevant for tissue-based work. Second, multiplexing greatly reduces sample preparation and instrument analysis time requirements, which is essential for the expanded application of proteomic analysis to translational medicine. Third, highly plexed analyses facilitate collection of biological replicate data, an obvious requisite for the field's advancement. Fourth, the ability to combine samples into a single analysis permits fewer MS experiments, which in turn allows for greater data overlap across all conditions. Finally, chemical labeling is compatible with biological tissues and fluids. We conclude the ability to analyze biological fluids and tissues is vital for the application of proteomics to translational medicine. In this TR&D project we aim to expand chemical labeling to an unprecedented 30-plex analysis, with the possibility to go much higher, by synthesis of new neutron-encoded chemical tags. Further, we will develop new technologies to boost MS/MS sampling overlap, since an MS/MS scan and identification is required to obtain quantitative data from isobaric tagging.

项目摘要 蛋白质定量技术的持续开发是现代生物学和医学的关键。 实际上，在定量分析过程中平行处理样品的能力（多重）对于许多 建议的DBP申请。通过化学标签的样品多路复用提供了许多关键优势。第一的， 单个质谱分析中样品的平行处理减少了所需的材料量 从任何一个样本中;这与基于组织的工作特别相关。第二，多路复用大大减少 样品准备和仪器分析时间要求，这对于扩展的应用至关重要 蛋白质组学分析到转化医学。第三，高度混音的分析有助于收集生物学 复制数据，这是该领域进步的明显必要条件。第四，将样品组合成一个的能力 单个分析允许更少的MS实验，这又允许在所有条件下更大的数据重叠。 最后，化学标记与生物组织和流体兼容。我们得出结论的分析能力 生物液和组织对于将蛋白质组学应用于转化医学至关重要。在此tr＆d中 我们旨在将化学标签扩展到前所未有的30质子分析，并有可能进行很多 更高，通过合成新的中子编码化学标签。此外，我们将开发新技术以提升 MS/MS采样重叠，因为需要MS/MS扫描和识别才能从中获取定量数据 等速标记。