A Zeiss Lightsheet Microscope for Rapid Multi-Dimensional Imaging

 描述（由申请人提供）：为了推进加州大学正在进行的NIH资助的研究，圣巴巴拉开发了Zeiss LightSheet Z. 1双面照明成像系统（LightSheet），用于实时或动态3D样本的快速、灵敏成像。拟议的文书不仅仅是对现有文书的改进（更好、更快、更敏感）。LightSheet是一种新型的商业仪器，能够对研究人员使用3D模型系统研究的动态事件进行成像。在探索升级和测试了众多系统后，研究人员发现LightSheet能够支持他们的研究项目。一些模型系统（例如海鞘，Botryllus schlosseri）不会在精心控制的环境之外发育，特别是当它们反复或连续暴露于强光下时。LightSheet显微镜技术通过分离激发和发射光路来限制光剂量。LightSheet仅照亮成像平面，发射的荧光通过单独的物镜收集。该仪器提供的时间和空间分辨率是目前研究的迫切需要。为了使仪器的最佳使用和长期维护，研究人员已经收集了大量的校园技术和财政支持。这一工具不仅有助于进行中的校园研究，而且有助于开发具有更广泛影响的资源。

 DESCRIPTION (provided by applicant): To advance the ongoing NIH funded research at UC, Santa Barbara a Zeiss LightSheet Z.1dual side illumination imaging system (LightSheet) for fast, sensitive imaging of live or dynamic 3D samples. The proposed instrument is not simply an improvement (better, faster, more sensitive) to an existing instrument. The LightSheet is a new type of commercial instrument that is uniquely capable of imaging the dynamic events these researchers study using 3D model systems. After exploring upgrades and testing numerous systems, the researchers found that the LightSheet was uniquely capable of supporting their research projects. Some model systems (e.g. Ascidians, Botryllus schlosseri) do not develop outside carefully controlled environments, particularly when they are repeatedly or continuously exposed to doses of high light. The LightSheet microscope technology limits the light dosage and by decoupling the excitation and emission light path. The LightSheet only illuminates the imaging plane and the emitted fluorescence is collected with a separate objective. The temporal and spatial resolution provided by this instrument are a critically need for ongoing research. To enable the best use and long-term maintenance of the instrument the researchers have gathered significant campus technical and financial support. The instrument would enable not only ongoing campus research but also the development of resources with broader impact.