A Zeiss Lightsheet Microscope for Rapid Multi-Dimensional Imaging

 描述(由申请者提供)：为了推进加州大学圣巴巴拉分校正在进行的由NIH资助的研究，一台蔡司LightSheet Z.1双面照明成像系统(LightSheet)用于快速、灵敏地对实时或动态3D样本进行成像。拟议的文书不仅仅是对现有文书的改进(更好、更快、更灵敏)。LightSheet是一种新型的商业仪器，它独特地能够成像研究人员使用3D模型系统研究的动态事件。在探索升级并测试了许多系统后，研究人员发现，LightSheet是唯一能够支持他们的研究项目的设备。一些模型系统(如海鞘、白背天牛)在严格控制的环境下不会发育，特别是当它们反复或持续暴露在高光剂量下时。LightSheet显微镜技术通过分离激发和发射光路来限制光量。光片只照亮成像平面，发射的荧光是用单独的物镜收集的。该仪器提供的时间和空间分辨率是正在进行的研究的迫切需要。为了使该仪器能够得到最好的使用和长期维护，研究人员已经收集了大量的校园技术和资金支持。该工具不仅将使正在进行的校园研究成为可能，而且还将使资源的开发产生更广泛的影响。

 DESCRIPTION (provided by applicant): To advance the ongoing NIH funded research at UC, Santa Barbara a Zeiss LightSheet Z.1dual side illumination imaging system (LightSheet) for fast, sensitive imaging of live or dynamic 3D samples. The proposed instrument is not simply an improvement (better, faster, more sensitive) to an existing instrument. The LightSheet is a new type of commercial instrument that is uniquely capable of imaging the dynamic events these researchers study using 3D model systems. After exploring upgrades and testing numerous systems, the researchers found that the LightSheet was uniquely capable of supporting their research projects. Some model systems (e.g. Ascidians, Botryllus schlosseri) do not develop outside carefully controlled environments, particularly when they are repeatedly or continuously exposed to doses of high light. The LightSheet microscope technology limits the light dosage and by decoupling the excitation and emission light path. The LightSheet only illuminates the imaging plane and the emitted fluorescence is collected with a separate objective. The temporal and spatial resolution provided by this instrument are a critically need for ongoing research. To enable the best use and long-term maintenance of the instrument the researchers have gathered significant campus technical and financial support. The instrument would enable not only ongoing campus research but also the development of resources with broader impact.