An integrated system to monitor complex tissues at single-cell resolution

以单细胞分辨率监测复杂组织的集成系统

• 批准号: 9114130
• 负责人: Zhirong Bao
• 金额: $ 49.82万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-24 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9114130
• 关键词: Adoption; Algorithms; Animal Model; Benchmarking; Biological; Brain; Cell Culture Techniques; Cells; Communities; Complex; Computer software; Computers; Congenital Abnormality; Data Set; Development; Disease; Ensure; Hand; Health; Image; Image Analysis; Individual; Lighting; Location; Measurement; Microscope; Microscopy; Monitor; Performance; Phototoxicity; Physiological; Population; Research; Resolution; Software Tools; Speed; System; Techniques; Technology; Time; Tissue imaging; Tissues; Translations; Validation; Virus Diseases; base; biological research; cell behavior; cost; experience; imaging software; improved; microscopic imaging; novel; prototype; public health relevance; research study; single cell analysis; tool; usability

DESCRIPTION (provided by applicant): We have recently developed a prototype system for long-term monitoring of single-cell behaviors in complex tissues. Our system comes from the synergistic integration and optimization of two leading technologies in microscopy and computational cell tracking: the inverted selective plane illumination microscope (iSPIM) and the StarryNite software package. We have demonstrated order-of-magnitude improvements on virtually all fronts compared to the standard technologies in use, notably imaging speed, reduction of phototoxicity, accuracy of cell tracking, and lower demand for computing power, all achieved at a reduced cost with comparable or better image quality. Our system has been applied to multiple model organisms as well as cell culture, demonstrating its versatility. We aim to bring the system to maturity and into the hands of the research community as a powerful and versatile tool for single-cell studies in complex, differentiating cell populations.

描述（由申请人提供）：我们最近开发了一种原型系统，用于长期监测复杂组织中的单细胞行为。我们的系统来自显微镜和计算细胞追踪领域两项领先技术的协同集成和优化：倒置选择性平面照明显微镜 (iSPIM) 和 StarryNite 软件包。与正在使用的标准技术相比，我们几乎在所有方面都实现了数量级的改进，特别是成像速度、光毒性的降低、细胞跟踪的准确性以及对计算能力的需求降低，所有这些都是以较低的成本实现的，并且具有可比或更好的图像质量。我们的系统已应用于多种模型生物以及细胞培养，展示了其多功能性。我们的目标是使该系统成熟并交到研究界手中，作为复杂、分化细胞群中单细胞研究的强大且多功能的工具。

项目成果

Using Stage- and Slit-Scanning to Improve Contrast and Optical Sectioning in Dual-View Inverted Light Sheet Microscopy (diSPIM).

• DOI: 10.1086/689589
• 发表时间: 2016-08
• 期刊: The Biological bulletin
• 作者: Kumar A;Christensen R;Guo M;Chandris P;Duncan W;Wu Y;Santella A;Moyle M;Winter PW;Colón-Ramos D;Bao Z;Shroff H
• 通讯作者: Shroff H