UMass Mouse Metabolic Phenotyping Center - Analytical and Functional Core

项目摘要-摘要 分析和功能核心的主要目标是进行标准化和专业化的实验 在清醒小鼠和衍生组织中进行小鼠模型的综合代谢表征， 肥胖、糖尿病及其并发症。核心由3个子核心组成，它们在本质上是互补的。的 分析子核心由Randall Friedline博士在胡女士的协助下指导，并提供以下内容 服务：1）激素的血清/组织/细胞培养物水平的高通量和多重测量， 细胞因子和趋化因子的高通量测量，2）细胞因子和趋化因子的高通量测量， 使用Cobas临床化学分析仪测定代谢物和电解质的血液/组织/尿液水平，3）分子 分析与胰岛素作用、葡萄糖代谢和炎症相关的细胞内信号通路， 和4）难以检测的分析物或在本发明中不可用的分析物的超灵敏单重ELISA测量。 多路复用格式。小岛子核心是共同指导博士.劳拉·阿隆索和戴尔Greiner，并提供 以下服务：1）使用H&E图像的胰岛结构，2）β细胞质量、增殖、死亡和大小，以及3） 测量静态、灌注和体内葡萄糖刺激的胰岛素分泌的胰岛功能。心血管 sub-Core由John Keaney博士和Timothy Fitzgibbons博士共同指导，并提供以下服务：1） 使用VisualSonics Vevo 2100 In Vivo Imaging的高分辨率二维和M型超声心动图 评估左心室收缩期尺寸和功能、左心室舒张期尺寸和 功能包括组织多普勒成像和心脏瓣膜功能，2）胸主动脉超声成像 血管形态学，3）使用CODA血压的血压和心率的非侵入性评估 监测系统，以及4）血管和内皮功能的评估。

Project Summary - Abstract The primary objective of the Analytical & Functional Core is to conduct standardized and specialized experiments in conscious mice and derived tissues for the comprehensive metabolic characterization of mouse models of obesity, diabetes and its complications. The Core consists of 3 sub-Cores that are complimentary in nature. The Analytical sub-Core is directed by Dr. Randall Friedline with assistance from Ms. Hu and provides the following services: 1) High-throughput and multiplexed measurement of serum/tissue/cell culture levels of hormones, cytokines, and chemokines using Luminex multiplexing technology, 2) High-throughput measurement of blood/tissue/urine levels of metabolites and electrolytes using a Cobas Clinical Chemistry Analyzer, 3) Molecular analysis of intracellular signaling pathways associated with insulin action, glucose metabolism, and inflammation, and 4) Ultra-sensitive single-plex ELISA measurements of difficult to detect analytes or those not available in multi-plex format. The Islet sub-Core is co-directed by Drs. Laura Alonso and Dale Greiner and provides the following services: 1) islet architecture using H&E images, 2) β-cell mass, proliferation, death, and size, and 3) islet function measuring static, perifusion, and in vivo glucose stimulated insulin secretion. The Cardiovascular sub-Core is co-directed by Drs. John Keaney and Timothy Fitzgibbons and provides the following services: 1) High-resolution two-dimensional and M-mode echocardiography using VisualSonics Vevo2100 In Vivo Imaging System to assess left ventricular systolic dimensions and function, left ventricular diastolic dimensions and function including tissue Doppler imaging, and cardiac valve function, 2) Ultrasound imaging of thoracic aorta vascular morphology, 3) Non-invasive assessment of blood pressure and heart rate using CODA blood pressure monitoring system, and 4) Assessment of vascular and endothelial function.