Novel Antigen Identification for an Enterotoxigenic E. coli Vaccine

产肠毒素大肠杆菌疫苗的新抗原鉴定

• 批准号: 9116756
• 负责人: James Michael Fleckenstein
• 金额: $ 44.29万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9116756
• 关键词: Address; Age; Animal Model; Antibody Response; Antigen Targeting; Antigens; Area; Bacterial Adhesins; Benchmarking; Biological Markers; Birth; Cessation of life; Child; Childhood; Clinical Trials; Communicable Diseases; Complex; DNA Sequence; Data; Developed Countries; Developing Countries; Development; Diarrhea; Disease Outbreaks; Escherichia coli; Escherichia coli Infections; Escherichia coli Vaccines; Fostering; Funding; Genetic; Genome; Genomics; Goals; Health; Human; Immune response; Immunity; Immunoassay; Immunologics; Infection; Infection prevention; Kinetics; Knowledge; Laboratories; Lead; Methods; Molecular; Mucins; Nature; Organism; Pathogenesis; Phase; Population Heterogeneity; Protein Microchips; Proteins; Proteome; Recombinants; Research Personnel; Sampling; Sanitation; Serine Protease; Small Intestines; Surface; Testing; Toxin; Translating; Traveler' s diarrhea; United States; Vaccine Antigen; Vaccine Design; Vaccines; Water; Work; base; cohort; design; enterotoxigenic Escherichia coli; extracellular; genome sequencing; global health; immunogenic; improved; microbial; mortality; next generation; novel; pan-genome; pathogen; pre-clinical; response; transcriptome; vaccine candidate; vaccine development; vaccinology

 DESCRIPTION (provided by applicant): This work focuses on enterotoxigenic Escherichia coli (ETEC), globally the most common bacterial cause of serious diarrheal illness. These illnesses threaten the lives of many children in poor regions of the world where sanitation and clean water are limited. While it has long been known that prior infections with these organisms offer protection against later infection, the nature of the protection is not known. The long-term goal o these studies is to address very basic questions that have eluded ETEC investigators trying to develop vaccines for these organisms: 1."To what proteins do infected people mount antibody responses after infection?" 2. "Which of these responses appear to be associated with protection against ETEC?" 3. "Can we show that these proteins trigger protective immune responses in a vaccine?" Studies to date have shown us that the immune response following ETEC infection is actually quite complicated, with recognition of many proteins. Some novel proteins that are not part of vaccines currently being tested could be added to improve the coverage and function of these vaccines. The basic goals of this proposal are to accelerate the discovery of novel protective proteins and to translate their use into effective ETEC vaccine components. Addressing these fundamental questions will fill important gaps in our understanding of protective immune responses that develop following ETEC infections, and permit a more rational approach to development of a broadly protective vaccine for these pathogens of global importance.

 描述（由申请人提供）：这项工作的重点是产肠毒素大肠杆菌（ETEC），这是全球最常见的严重腹泻疾病的细菌原因。这些疾病威胁着世界上卫生设施和清洁水有限的贫困地区许多儿童的生命。虽然人们早就知道，以前感染这些生物体可以防止后来的感染，但这种保护的性质尚不清楚。这些研究的长期目标是解决ETEC研究人员试图为这些生物体开发疫苗时遇到的非常基本的问题：1。“感染者在感染后对哪些蛋白质产生抗体反应？“2.“这些反应中哪些似乎与ETEC的保护有关？“3.“我们能证明这些蛋白质在疫苗中引发保护性免疫反应吗？“迄今为止的研究表明，ETEC感染后的免疫反应实际上非常复杂，可以识别许多蛋白质。可以添加一些目前正在测试的疫苗中没有的新蛋白质，以提高这些疫苗的覆盖率和功能。该提案的基本目标是加速发现新的保护性蛋白，并将其转化为有效的ETEC疫苗组分。解决这些基本问题将填补我们对ETEC感染后产生的保护性免疫反应的理解中的重要空白，并允许更合理的方法来开发针对这些全球重要病原体的广泛保护性疫苗。