Loyola Project

新出现的证据表明，膀胱并不像以前认为的那样无菌。这一证据还表明， 常驻细菌群落，女性泌尿微生物组（FUM），可能影响多种泌尿系统疾病， 包括急迫性尿失禁、尿道感染和可能的泌尿系统疼痛综合征。的 健康女性的尿液细菌来源可能为了解细菌引起或 泌尿系统疾病的贡献。随着我们对以下方面的了解的深入，我们预计会产生重大的临床影响： 可以量化、预测、预防和/或改变的FUM生态失调。为了增进这方面的知识，我们 申请资金来检验FUM受相邻盆腔微生物组影响的总体假设（即， 阴道和会阴），并且FUM成分与泌尿系统症状和结果密切相关。 我们建议描述一个预先存在的尿液样本和阴道及会阴拭子库， 来自300多名特征鲜明的成年女性。利用16 S rRNA序列分析和创新的生物信息学 分析，我们将表征这些样品中的细菌群落，以研究 FUM和相邻骨盆龛的微生物组（目标1），以及这些微生物组中的每一个之间， 临床结果（目标2）。这一信息将迅速与更广泛的泌尿外科研究界共享 通过一个专门的基于网络的数据库（目标3），促进对不同临床状况的进一步研究， 产生未来的假设。

Emerging evidence shows that the bladder is not sterile as previously thought. This evidence also shows that the resident bacterial community, the female urinary microbiome (FUM), may impact multiple urinary disorders, including urgency urinary incontinence, urinary tract infections and possibly urologic pain syndromes. The origins of urinary bacteria in healthy women may provide important insights into the bacterial cause of or contribution to urinary disorders. We anticipate major clinical implications as we advance knowledge concerning FUM dysbioses that can be quantified, predicted, prevented and/or modified. To advance that knowledge, we request funds to test the overarching hypothesis that the FUM is influenced by adjacent pelvic microbiomes (i.e., vaginal and perineal) and that FUM compositions are strongly associated with urinary symptoms and outcomes. We propose to characterize a pre-existing repository of urine samples and vaginal and perineal swabs obtained from >300 well-characterized adult women. Using 16S rRNA sequence analysis and innovative bioinformatics analysis, we will characterize the bacterial communities in these samples to investigate relationships between the FUM and the microbiomes of adjacent pelvic niches (Aim 1), and between each of these microbiomes and clinical findings (Aim 2). This information will be promptly shared with the wider urological research community via a dedicated web-based database (Aim 3), facilitating further study of diverse clinical conditions, and generating future hypotheses.