Functional and structural dissection of the human replisome

该项目旨在开发可视化人类复制体的结构和酶活性的技术，复制体是在细胞分裂前复制DNA的多蛋白组装。新的单分子和最先进的冷冻电子显微镜的组合将被用来定义人类复制体如何在复制压力期间协调DNA合成。该项目的主要成果包括开发新的分子可视化技术，导致首次对人类复制体使用的动态过程进行分子描述。好处包括提高对癌症中经常发生故障的基本生物过程的理解，开发新的方法学，以及跨学科培训。

This project aims to develop technology to visualise the structure and enzymatic activities of the human replisome, the multiprotein assembly that copies DNA before cell division. A combination of novel single-molecule and state-of-the-art cryo-electron microscopy will be used to define how the human replisome coordinates DNA synthesis during times of replication stress. Key outcomes of this project include development of novel molecular visualisation technologies, leading to the first molecular description of dynamic processes used by the human replisome. Benefits include improved understanding of a fundamental biological process that often malfunctions in cancers, development of novel methodology, and interdisciplinary training.