Towards a molecular fingerprint for human-specific endogenous retroviruses

人类特异性内源性逆转录病毒的分子指纹

• 批准号: DE240100707
• 负责人: Dr Dezerae Cox
• 金额: $ 31.26万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2024
• 资助国家: 澳大利亚
• 起止时间: 2024-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE240100707
• 关键词: Towards; molecular; fingerprint; human; specific

This project aims to understand how ancient viral sequences resident in the human genome can contribute to cellular processes. Using a novel molecular toolbox that combines affinity-directed proximity labelling mass spectrometry and single molecule microscopy, this project will characterise the cellular fingerprint of a human endogenous retrovirus family HERV-K (HML-2). This fingerprint will comprehensively describe how expressed HERV-K loci engage with the homeostasis network in human cells. This will provide significant benefits in the form of new knowledge concerning fundamental aspects of cellular homeostasis, and a state-of-the-art molecular biology toolbox ready to explore quantitatively the role of HERV-K in human health and disease.

该项目旨在了解驻留在人类基因组中的古老病毒序列如何对细胞过程做出贡献。使用结合亲和定向邻近标记质谱仪和单分子显微镜的新型分子工具箱，该项目将描述人类内源性逆转录病毒家族HERV-K(HML-2)的细胞指纹。这个指纹将全面描述表达的Herv-K基因座如何与人类细胞中的动态平衡网络相结合。这将以关于细胞动态平衡的基本方面的新知识的形式提供显著的好处，并提供一个最先进的分子生物学工具箱，准备好定量地探索HERV-K在人类健康和疾病中的作用。