Integrated free radical/nitric oxide analyzer

集成自由基/一氧化氮分析仪

• 批准号: 346137-2007
• 负责人: Arul, Joseph
• 金额: $ 1.65万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325605
• 关键词: Integrated; free; radical; nitric; oxide

The proposal for a RTI grant is intended to purchase an Integrated Free Radical / Nitric Oxide Analyzer, which employs electrochemical (amperometric) principle for detection of redox-reactive species with high accuracy and sensitivity. It relates to the applicant's Discovery grant award (2003-2008, Plant Biology and Food Science), entitled "Photochemical preventive therapy for the preservation of fresh fruits and vegetables by induced resistance and delayed ripening". The applicant's research program in postharvest biology aims at establishing the physiological basis of induced resistance and delayed senescence responses of the postharvest tissue by hormic doses of UV radiation (hormesis is the stimulation of beneficial responses by low levels of stressors which are otherwise harmful). From a practical standpoint, it aims at exploiting the potential of UV-hormesis effectively as a practical means of preservation for fresh fruits and vegetables. Our pioneering work has shown that hormic UV treatment not only builds disease resistance in the produce but also delays ripening. To develop UV treatment as a practical means of fresh fruits and vegetables preservation, a fundamental understanding of the biological phenomena of UV-induced disease resistance and delayed ripening is required. A major aspect of this research is to gain an understanding of the molecular events that occur in the tissue following UV treatment. Several signal molecules including redox signals (H2O2 and NO), and hormonal signals salicylic acid, jasmonic acid, ABA, ethylene and Ca2+ have been implicated in the modulation of plant responses to both abiotic and biotic stresses; and they are important in converting external signals into cellular response. Thus this research involves the characterization of stresses for their oxidative stress intensity and the determination of profiles of redox signals (H2O2 and NO). The Integrated Free Radical / Nitric Oxide Analyzer will be a valuable working tool for our research involving various stresses and interaction between them. It allows us to assay H2O2, NO as well as oxygen simultaneously in a given sample. A ready accessibility to the proposed equipment will improve productivity and progress of our research.

RTI 拨款提案旨在购买集成自由基/一氧化氮分析仪，该分析仪采用电化学（电流分析）原理以高精度和高灵敏度检测氧化还原反应物质。它涉及申请人的发现资助奖（2003-2008，植物生物学和食品科学），题为“通过诱导抗性和延迟成熟来保存新鲜水果和蔬菜的光化学预防疗法”。申请人在采后生物学方面的研究计划旨在建立采后组织通过毒物剂量的紫外线辐射诱导抵抗和延迟衰老反应的生理学基础（毒物兴奋作用是通过低水平的应激源刺激有益反应，否则这些应激物是有害的）。从实用的角度来看，它的目的是有效地利用紫外线兴奋作用的潜力，作为新鲜水果和蔬菜保鲜的实用手段。我们的开创性工作表明，激素紫外线处理不仅可以增强农产品的抗病性，还可以延迟成熟。为了将紫外线处理发展为新鲜水果和蔬菜保鲜的实用手段，需要对紫外线诱导的抗病性和延迟成熟的生物学现象有基本的了解。这项研究的一个主要方面是了解紫外线治疗后组织中发生的分子事件。包括氧化还原信号（H2O2 和 NO）和激素信号（水杨酸、茉莉酸、ABA、乙烯和 Ca2+）在内的多种信号分子与植物对非生物和生物胁迫反应的调节有关；它们对于将外部信号转化为细胞反应非常重要。因此，这项研究涉及氧化应激强度的应激表征以及氧化还原信号（H2O2 和 NO）分布的测定。集成自由基/一氧化氮分析仪将成为我们涉及各种压力及其相互作用的研究的宝贵工作工具。它使我们能够同时测定给定样品中的 H2O2、NO 以及氧气。方便地使用所提议的设备将提高我们研究的生产力和进展。