Integrated free radical/nitric oxide analyzer

RTI拨款的建议旨在购买一台综合自由基/一氧化氮分析仪，该分析仪采用电化学(安培)原理，以高精度和高灵敏度检测氧化还原反应物种。它涉及申请人的发现奖助金(2003-2008年，植物生物学和食品科学)，题为“通过诱导抗性和延迟成熟来保存新鲜水果和蔬菜的光化学预防疗法”。申请人的采后生物学研究计划旨在建立采后组织通过高剂量紫外线辐射诱导抗性和延迟衰老反应的生理学基础(兴奋作用是通过低水平的应激刺激有益的反应，否则是有害的)。从实用的角度来看，它的目的是有效地开发紫外线刺激作为一种实用的新鲜水果和蔬菜保鲜手段的潜力。我们的开创性工作表明，荷尔蒙紫外线处理不仅能增强农产品的抗病能力，还能延缓成熟。为了发展紫外线处理作为一种实用的新鲜水果和蔬菜保鲜手段，需要对紫外线诱导的抗病和延迟成熟的生物学现象有一个基本的了解。这项研究的一个主要方面是了解紫外线治疗后组织中发生的分子事件。一些信号分子，包括氧化还原信号(H_2O_2和NO)，以及激素信号水杨酸、茉莉酸、脱落酸、乙烯和钙离子，都参与了植物对非生物和生物胁迫的反应的调节；它们在将外部信号转化为细胞反应中起着重要的作用。因此，这项研究涉及对其氧化应激强度的压力的表征，以及氧化还原信号(H_2O_2和NO)轮廓的确定。集成的自由基/一氧化氮分析仪将是一个有价值的工作工具，我们的研究涉及各种压力及其相互作用。它允许我们同时检测给定样本中的过氧化氢、一氧化氮和氧气。方便地使用拟议的设备将提高我们的研究效率和进度。

The proposal for a RTI grant is intended to purchase an Integrated Free Radical / Nitric Oxide Analyzer, which employs electrochemical (amperometric) principle for detection of redox-reactive species with high accuracy and sensitivity. It relates to the applicant's Discovery grant award (2003-2008, Plant Biology and Food Science), entitled "Photochemical preventive therapy for the preservation of fresh fruits and vegetables by induced resistance and delayed ripening". The applicant's research program in postharvest biology aims at establishing the physiological basis of induced resistance and delayed senescence responses of the postharvest tissue by hormic doses of UV radiation (hormesis is the stimulation of beneficial responses by low levels of stressors which are otherwise harmful). From a practical standpoint, it aims at exploiting the potential of UV-hormesis effectively as a practical means of preservation for fresh fruits and vegetables. Our pioneering work has shown that hormic UV treatment not only builds disease resistance in the produce but also delays ripening. To develop UV treatment as a practical means of fresh fruits and vegetables preservation, a fundamental understanding of the biological phenomena of UV-induced disease resistance and delayed ripening is required. A major aspect of this research is to gain an understanding of the molecular events that occur in the tissue following UV treatment. Several signal molecules including redox signals (H2O2 and NO), and hormonal signals salicylic acid, jasmonic acid, ABA, ethylene and Ca2+ have been implicated in the modulation of plant responses to both abiotic and biotic stresses; and they are important in converting external signals into cellular response. Thus this research involves the characterization of stresses for their oxidative stress intensity and the determination of profiles of redox signals (H2O2 and NO). The Integrated Free Radical / Nitric Oxide Analyzer will be a valuable working tool for our research involving various stresses and interaction between them. It allows us to assay H2O2, NO as well as oxygen simultaneously in a given sample. A ready accessibility to the proposed equipment will improve productivity and progress of our research.