Enhancement of health-promoting and plant-protecting secondary metabolites by postharvest conditioning with hormetic doses of abiotic stresses

通过采用毒物兴奋剂剂量的非生物胁迫进行采后调节，增强促进健康和保护植物的次生代谢产物

• 批准号: 38284-2011
• 负责人: Arul, Joseph
• 金额: $ 2.91万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=568810
• 关键词: Enhancement; health; promoting; plant; protecting

Our long-term goal is to develop post-harvest conditioning technologies to enhance health-promoting (nutraceutical) and plant-protecting (phytoalexin) secondary metabolites in fresh fruits and vegetables using hormetic doses of abiotic stresses that are suitable for application in the postharvest phase. Given that plants produce secondary metabolites as weapons to combat stresses, it is possible that the response can well be tuned to achieve a balance between enhancement of dietary phytochemicals that offer plant protection and health benefits and acceptability of the produce by exposing them to controlled doses of stressors. This would require a better understanding of the link between stressors and secondary metabolites, and an understanding of the stress-induced alterations in the secondary metabolites and metabolic pathways. Thus our efforts in the short- to medium-term will be focused on gaining an understanding of the connections between hormetic doses of abiotic stressors, signal components and secondary metabolite production. The research involves: (1) the determination of hormetic doses of abiotic stresses in selected crops; (2) the selection of hormetic stresses which are effective in inducing or enhancing desired secondary metabolites without affecting sensory quality of the produce; (3) the characterization of the selected abiotic stresses and their combinations for their generation of redox and hormonal signals in the selected crops; (4) the profiling of secondary metabolites of the pathways that produce the desired phytochemicals in the crops exposed to the selected stresses and their combinations; and (5) the establishment of correlative relationships between signal components and secondary metabolites which will help identify key signal component(s) that impact the production of desired phytochemicals. Studies will be performed with carrot root and tomato fruit. Phytochemical-enriched fresh fruits and vegetables have recently been identified as a new class of functional foods. Preservation of fresh crops with enhanced attributes of health-promotion and disease resistance will not only help the fresh produce industry to reduce postharvest losses, but also help them to ensure a healthier population.

我们的长期目标是开发采后调理技术，利用适合采后阶段应用的毒效剂量的非生物胁迫来增强新鲜水果和蔬菜中促进健康（营养）和植物保护（植物抗毒素）的次生代谢产物。鉴于植物产生次生代谢物作为对抗压力的武器，因此可以很好地调整反应，以在增强膳食植物化学物质（提供植物保护和健康益处）和通过将产品暴露于受控剂量的压力源下使产品可接受性之间取得平衡。这需要更好地了解应激源和次级代谢物之间的联系，以及应激引起的次级代谢物和代谢途径的变化。因此，我们中短期的努力将集中于了解非生物应激源的激效剂量、信号成分和次级代谢产物产生之间的联系。研究内容包括：（1）确定选定作物中非生物胁迫的激效剂量； (2) 选择能有效诱导或增强所需次级代谢产物而不影响产品感官质量的激效应激； (3) 所选非生物胁迫及其组合在所选作物中产生氧化还原和激素信号的特征； (4) 对暴露于选定胁迫及其组合的作物中产生所需植物化学物质的途径的次生代谢物进行分析； (5) 建立信号成分和次生代谢物之间的相关关系，这将有助于识别影响所需植物化学物质产生的关键信号成分。将以胡萝卜根和番茄果实为对象进行研究。富含植物化学物质的新鲜水果和蔬菜最近被确定为一类新的功能性食品。增强健康促进和抗病能力的新鲜作物的保存不仅有助于新鲜农产品行业减少采后损失，还有助于确保人口健康。