Spectrometer for microbiology and protein studies

用于微生物学和蛋白质研究的光谱仪

• 批准号: 344912-2007
• 负责人: Turner, Raymond
• 金额: $ 1.89万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326036
• 关键词: Spectrometer; microbiology; protein; studies

Spectrophotometers are a key instrument in Life Science laboratories and are used to evaluate, identify, and characterize molecules and cells.  Our research group studies resistance to antimicrobial compounds including antiseptics, DNA inter-chelating Dyes and heavy metals. As the dyes are fluorescent and their fluorescence changes when inside the cell versus on the outside, we can perform transport assays using the fluorescent detection mode of the spectrometer.   Using metal chelating compounds using the fiber optic mode of the spectrometer we will be able to assess concentrations of metals within bacterial biofilms.   We also study proteins that act as chaperones or escorts of other proteins in the cell and we are interested in this recognition and interaction process. We have developed an assay that explores how a chaperone protein interacts with a peptide. Finally, we are developing novel chemistries towards proteins using photochemical reactions. We separate the reaction products by chromatography and we will use the spectrometer with an in line detection to identify interesting products for further investigations.  Overall the spectrometer requested will be used for several hours a day by more over 15 researchers and research trainees.

分光光度计是生命科学实验室的关键仪器，用于评估，识别和表征分子和细胞。我们的研究小组研究抗菌化合物的耐药性，包括防腐剂，DNA间螯合染料和重金属。由于染料是荧光的，并且它们的荧光在细胞内与细胞外时发生变化，因此我们可以使用光谱仪的荧光检测模式进行转运测定。 使用光谱仪的光纤模式使用金属螯合化合物，我们将能够评估细菌生物膜内的金属浓度。 我们还研究蛋白质作为伴侣或护送其他蛋白质在细胞中，我们感兴趣的是这种识别和相互作用的过程。我们已经开发了一种检测方法，探索伴侣蛋白如何与肽相互作用。最后，我们正在利用光化学反应开发新的蛋白质化学。我们通过色谱法分离反应产物，并将使用在线检测的光谱仪来识别感兴趣的产物，以进行进一步的研究。总的来说，超过15名研究人员和研究实习生每天将使用所要求的光谱仪数小时。