Beyond Spectral Detection: Engineering SUPER Dot Probes for High-Throughput Discovery

超越光谱检测：设计超级点探针以实现高通量发现

• 批准号: FT130100517
• 负责人: Prof Dayong Jin
• 金额: $ 50.88万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-03-03 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT130100517
• 关键词: Beyond; Spectral; Detection; Engineering; SUPER

Molecules that are altered as a result of a pathological condition are generally present in very low abundance, and pose a “needle-in-a-haystack” problem. Current detection, quantification and localisation technologies use fluorescent probes that are limited by sensitivity and analysis time. This project will develop a new generation of nanophotonic luminescent probes (Strong Upconversion Photo-stable Encoded nano-Radiators (SUPER) Dots), based on purpose-engineered up-conversion nanocrystals that are ultra-bright and have low background interference, high specificity, speed, and large-scale multiplexing capacity. These probes will allow microscopy and flow cytometry to measure hitherto undetectable rare-event molecules and cells, opening new frontiers for the discovery of new biomarkers.

由于病理情况而改变的分子通常以非常低的丰度存在，并构成一个大海捞针的问题。目前的检测、定量和定位技术使用的荧光探针受到灵敏度和分析时间的限制。该项目将开发新一代纳米光子发光探测器(强上转换光稳定编码纳米辐射体(Super)Dots)，基于专门设计的上转换纳米晶体，具有超亮、低背景干扰、高特异性、高速度和大规模复用能力。这些探针将允许显微镜和流式细胞仪测量迄今为止无法检测到的罕见事件分子和细胞，为发现新的生物标记物开辟新的前沿。