Regulation of slow troponin C gene expression

慢肌钙蛋白 C 基因表达的调节

• 批准号: 3542-2006
• 负责人: Bag, Jnanankur
• 金额: $ 3.38万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=337372
• 关键词: Regulation; slow; troponin; gene; expression

Decoding of the genetic information involves the synthesis of messenger RNA(mRNA)from the DNA.This process takes place in the nucleus of mammalian cells.The mRNA after exiting the nucleus is translated in the cytoplasm to produce proteins,which are required for all cellular functions.Recent studies suggests that mRNAs are sorted to different cytoplasmic locations.This process is believed to be important for assymatric distribution of proteins in the cell. Assymatric distribution of proteins  is an  important aspect of proper embryonic development.Therefore, expression of a protein at the wrong place in the cell could be detrimental and may result in the altered body plan of the embryo.Trafficking of mRNAs to its proper cytoplasmic location is also important for the assembly of many multicomponent cellular structures,such as the muscle filaments.If the mRNAs encoding different proteins of the muscle filament are co-localized at a distinct cytoplasmic site,expression of all protein components at the same site, would be more efficient for their assembly into the muscle structure,than relying on the simple diffusion process.How mRNAs are sorted to different locations in the cytoplasm is still not clear.We are currently investgating whether mRNAs contain their own zipcode.Our studies on an mRNA that encodes one of the muscle proteins,the troponin C, showed that it has a zipcode for loclizing it at the perinuclear site.We are further characterizing how this zip code anchors the mRNA at its site. We propose that it binds to a specific zipcode binding protein at the perinuclear cytoplasmic tract.Therefore we plan to identify the troponin C mRNA's zipcode binding protein and determine how it functions in muscle cells. Our work is expected to produce new knowledge to improve our understanding of the important cellular process of mRNA trafficking.

遗传信息的解码涉及到从DNA合成信使RNA（mRNA）。这个过程发生在哺乳动物的细胞核中。mRNA离开细胞核后在细胞质中翻译产生蛋白质，这些蛋白质是所有细胞功能所必需的。最近的研究表明，mrna被分类到不同的细胞质位置。这一过程被认为对细胞内蛋白质的不对称分布很重要。蛋白质的不对称分布是胚胎正常发育的一个重要方面。因此，在细胞中错误的位置表达蛋白质可能是有害的，并可能导致胚胎身体计划的改变。mrna转运到其适当的细胞质位置对于许多多组分细胞结构（如肌丝）的组装也很重要。如果编码肌丝不同蛋白质的mrna在不同的细胞质位点共定位，那么在同一位点表达所有蛋白质成分，将比依赖简单的扩散过程更有效地将其组装到肌肉结构中。mrna如何在细胞质中被分类到不同的位置尚不清楚。我们目前正在调查mrna是否包含自己的邮政编码。我们对一种编码肌钙蛋白C的mRNA的研究表明，它有一个邮政编码，可以定位在核周位置。我们将进一步描述这个邮政编码是如何将mRNA锚定在其位置上的。我们提出，它结合到一个特定的邮政编码结合蛋白在核周细胞质束。因此，我们计划鉴定肌钙蛋白C mRNA的邮编结合蛋白，并确定其在肌肉细胞中的功能。我们的工作有望产生新的知识，以提高我们对mRNA运输的重要细胞过程的理解。