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Insertion of bacteria type III secretion proteins into target cell membrane

将细菌 III 型分泌蛋白插入靶细胞膜

基本信息

批准号：
238249-2006
负责人：
Jardim, Armando
金额：
$ 2.2万
依托单位：
McGill University
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2007
资助国家：
加拿大
起止时间：
2007-01-01 至 2008-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362039
关键词：
Insertion bacteria type III secretion

项目摘要

Enteropathogenic and enterohemorrhagic E. coli are responsible for a vast number of food and water borne gastrointestinal diseases that are characterized by severe and persistent diarrhea. These bacteria contain a unique group of virulence proteins called the type III secretion system (TTS). The TTSS consists of two classes of bacterial protein designated translocator and effector proteins. The translocator proteins are involved in forming a molecular tunnel called a needle complex that grows out from the bacterial cell to make contact with the epithelial cells that line the intestinal tract. Once the needle complex docks with the epithelial cell surface the bacteria release a set of called the pore forming proteins that penetrate the plasma membrane of the host cell allowing the bacteria to inject the effector proteins directly into the epithelial cell. These effector proteins act to modify both the structure and cellular function of the epithelial cell to allow bacteria to attach and colonize the intestine. A key aspect of this process that is not well understood is how the bacterial pore forming proteins selectively penetrate the epithelial cell membrane. The aim of this proposal is to study is to examine the molecular event involve in formation of the membrane pore that permits bacterial proteins to be translocated directly into the epithelial cell.

肠致病性和肠出血性E.大肠杆菌是导致大量以严重和持续腹泻为特征的食源性和水源性胃肠道疾病的原因。这些细菌含有一组称为III型分泌系统（TTS）的独特毒力蛋白。TTSS由两类细菌蛋白质组成，称为转运蛋白和效应蛋白。转运蛋白参与形成称为针复合物的分子通道，从细菌细胞中生长出来，与排列在肠道中的上皮细胞接触。一旦针复合物与上皮细胞表面对接，细菌释放一组称为孔形成蛋白的蛋白质，其穿透宿主细胞的质膜，允许细菌将效应蛋白直接注射到上皮细胞中。这些效应蛋白的作用是改变结构和细胞上皮细胞的功能，使细菌附着和殖民肠道。这一过程的一个关键方面是没有得到很好的理解是细菌孔形成蛋白如何选择性地穿透上皮细胞膜。本研究的目的是检测膜孔形成过程中的分子事件，该膜孔允许细菌蛋白直接转运到上皮细胞中。