Upgrade of the Lakehead instrument laboratory GC-MS instrument for biotechnology research

升级 Lakehead 仪器实验室 GC-MS 仪器用于生物技术研究

• 批准号: 360361-2008
• 负责人: Law, David
• 金额: $ 6.18万
• 依托单位: Lakehead University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362228
• 关键词: Upgrade; Lakehead; instrument; laboratory; GC

Gas chromatography-mass spectrometry (GC-MS) is a method of identifying low levels of compounds produced during a manufacturing process or that are present in the environment, plant tissues, or animal tissues, including the human body. These molecules may be important for better understanding how to make higher value materials with unique properties, how genes regulate the growth and development of plants and animals, and how low levels of some environmental contaminants exert a profound effect on human health. GC-MS is a technology that allows researchers to identify and quantify these molecules. The existing GC-MS at Lakehead University can separate and resolve components from complex mixtures, but is limited in its sensitivity. This means that a large starting sample must be used in order to detect compounds. However, many biological and chemical samples are small, for example, the small number of human cells extracted during a biopsy, or micrograms of product synthesized during a chemical reaction in a research laboratory. By upgrading this instrument from a low sensitivity single quadrupole to a triple quadrupole, the GC-MS will then have a ten-fold or more increase in outright sensitivity, allowing the use of much smaller samples for analysis. Additionally, the upgrade will provide more accurate data because the peaks given off by the ions produced during the passage of the compound through the instrument are better resolved. This also allows accurate quantification of compounds, which is necessary for the development of new medical testing procedures for disease detection. The upgraded instrument will support a broad range of research at Lakehead and significantly boost the quality and quantity of research generated by chemists, biologists, and medical scientists at the University. The upgraded GC-MS will allow the training of young scientists at Lakehead in a quantitiative technique that is in very high demand in Canadian industries, such as pharmaceutical research, biotechnology and biochemistry. The higher resolution GC-MS is critical for advancing environmental and medical biotechnology research that is beginning to flourish at Lakehead.

气相色谱-质谱仪(GC-MS)是一种识别制造过程中产生的或存在于环境、植物组织或动物组织(包括人体)中的低水平化合物的方法。这些分子可能对于更好地理解如何制造具有独特性能的高价值材料，基因如何调控动植物的生长发育，以及一些环境污染物的低水平如何对人类健康产生深远影响，可能是重要的。GC-MS是一种允许研究人员识别和量化这些分子的技术。莱克黑德大学现有的GC-MS可以从复杂混合物中分离和分解组分，但其灵敏度有限。这意味着为了检测化合物，必须使用较大的起始样品。然而，许多生物和化学样本都很小，例如，在活组织检查中提取的少量人体细胞，或在研究实验室的化学反应中合成的微克产品。通过将该仪器从低灵敏度的单个四极杆升级为三个四极杆杆，GC-MS的完全灵敏度将提高十倍或更多，从而允许使用小得多的样品进行分析。此外，升级将提供更准确的数据，因为在化合物通过仪器时产生的离子发出的峰得到更好的分辨。这还可以对化合物进行准确的量化，这对于开发新的疾病检测医学测试程序是必要的。升级后的仪器将支持莱克黑德大学的广泛研究，并显著提高该大学化学家、生物学家和医学科学家产生的研究的质量和数量。升级后的GC-MS将允许对莱克黑德的年轻科学家进行量化技术方面的培训，这种技术在加拿大的制药研究、生物技术和生物化学等行业中需求非常高。更高分辨率的GC-MS对于推动环境和医学生物技术研究至关重要，这些研究正开始在莱克黑德蓬勃发展。