Equipment for behavioural phenotyping of mice

小鼠行为表型分析设备

• 批准号: 359657-2008
• 负责人: Brown, Richard
• 金额: $ 1.13万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362533
• 关键词: Equipment; behavioural; phenotyping; mice

In order to conduct our experiments on behavioral phenotyping of mice we require three sets of equipment.The Tonolab measures the intra-optic pressure of rats and mice. It is used to detect glaucoma, which results in visual deficits. We require this equipment for the PhD thesis of Aimee Wong.We always videotape our behavioural studies so that the behaviour can be rescored by a second observer to ensure reliability and so that we can identify any experimenter errors. We have used videotapes but because of the digital revolution, it is becoming difficult to purchase blank videotapes. It also takes a lot of room to store them. Thus we are required to use digital video cameras and computers with larger amounts of storage and DVD burners. Our present video system and computers are over 10 years old and therefore must be replaced. This equipment is necessary for Timothy O'Leary's PhD research.We have developed a battery of tests for anxiety, learning and memory in mice, but have no test system for avoidance conditioning. Because many transgenic mice have social and affective disorders, we also need learning tasks that involve social behavior, such as the social transmission of food preference task. In order to remove brains from mice and perform perfusions, we require new surgery equipment. We now use equipment purchased by Shinshu Nakajima in the 1970's, and much of it needs to be replaced. We are also completing immuno-histochemistry on the brains of transgenic mice, which requires a shaker platform to prepare the antibodies. This equipment is necessary for Leanne Fraser's MSc research.

为了进行小鼠行为表型的实验，我们需要三套设备。Tonolab测量大鼠和小鼠的视内压。它被用来检测青光眼，青光眼会导致视力下降。我们需要这个设备用于Aimee Wong的博士论文。我们总是把我们的行为研究录下来，这样第二个观察者就可以重新记录行为，以确保可靠性，这样我们就可以识别任何实验错误。我们曾经使用过录像带，但由于数字革命，购买空白录像带变得越来越困难。储藏它们也需要很大的空间。因此，我们需要使用数字摄像机和具有更大存储容量的计算机以及DVD刻录机。我们目前的视频系统和电脑已经使用了10年以上，因此必须更换。这个设备是蒂莫西·奥利里博士研究的必需品。我们已经在老鼠身上开发了一系列的焦虑、学习和记忆测试，但还没有针对回避条件反射的测试系统。由于许多转基因小鼠存在社会和情感障碍，我们还需要涉及社会行为的学习任务，如食物偏好的社会传递任务。为了从老鼠身上取出大脑并进行灌注，我们需要新的手术设备。我们现在使用的是1970年代中岛信书购买的设备，其中大部分需要更换。我们也正在完成转基因小鼠大脑的免疫组织化学，这需要一个振动平台来制备抗体。这个设备是Leanne Fraser的硕士研究所必需的。