Physiological roles of pro-oxidants/antioxidants in the bovine female tract

促氧化剂/抗氧化剂在牛雌性肠道中的生理作用

• 批准号: 238570-2007
• 负责人: Bilodeau, JeanFrançois
• 金额: $ 2.25万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364123
• 关键词: Physiological; roles; pro; oxidants; antioxidants

Reproductive success during early pregnancy relies on complex and progressive interactions between the maternal genital tract, gametes, fertilised oocytes and embryos. Our basic research interest is to understand the roles of reactive oxygen species (ROS : nitric oxide (NO), hydrogen peroxide (H2O2) etc..) and their neutralisation by antioxidant systems in female reproductive functions. The physiological roles in the female tract of ROS and antioxidant defenses are poorly understood and it is our global goal to understand these roles. The first objective of the research program is to determine 1) which ROS or antioxidant defenses are involved in the physiological process of fertilisation and embryo implantation? 2) How ROS and antioxidants are regulated in the female tract in relation to the physiological events of fertilisation and implantation? 3) How ROS and antioxidant defenses affect prostaglandins biosynthesis? Prostaglandins are involved in many physiological events in reproduction. The study of the expression of ROS generators and antioxidant enzymes in the bovine female tract according to the physiologic status has first been investigated by us. This project requires the analysis of protein and genetic expression in the uterus and the oviduct of the cow. Investigation of the type of cells involved is also an important objective. In vitro studies with uterine and oviductal cells are also planned. This research will lead to a better understanding of the physiology of reproduction of a wide spread domestic animal in Canada, the cow. This research will be tentatively transpose or eventually compared to other species. This research can also help us to diagnose infertility and finally help us to improve fertility in domestic animals.

妊娠早期的生殖成功依赖于母体生殖道、配子、受精卵母细胞和胚胎之间复杂而渐进的相互作用。我们的基本研究兴趣是了解活性氧（ROS：一氧化氮（NO），过氧化氢（H2O2）等）的作用。以及它们在女性生殖功能中被抗氧化系统中和。ROS和抗氧化防御在女性体内的生理作用知之甚少，我们的全球目标是了解这些作用。该研究计划的第一个目标是确定1）哪些ROS或抗氧化剂防御参与受精和胚胎植入的生理过程？2)活性氧和抗氧化剂如何在女性生殖道中与受精和着床的生理事件有关？3)ROS和抗氧化剂防御如何影响拟南芥素的生物合成？前列腺素参与生殖过程中的许多生理事件。本研究首次对牛雌性生殖道中活性氧产生物和抗氧化酶的表达与生理状态的关系进行了研究。该项目需要分析奶牛子宫和输卵管中的蛋白质和基因表达。研究所涉及的细胞类型也是一个重要的目标。还计划对子宫和输卵管细胞进行体外研究。这项研究将导致更好地了解繁殖的生理学在加拿大广泛传播的家畜，牛。这项研究将暂时转置或最终与其他物种进行比较。这项研究还可以帮助我们诊断不孕症，最终帮助我们提高家畜的生育能力。