Molecular tagging to measure two-dimensional fluid velocity in microchannels

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The equipment requested in this proposal offers a unique opportunity in the development of research in micro-fluidics.  Flow of liquid within micro-channels can be driven by pressure or electrical forces.  Characterizing this fluid motion experimentally is very challenging.  Typical techniques monitor the motion of particles seeded in the flow.  These particles however may not follow true fluid motion especially if the particles are of a size similar to the channel width.  Experimental data is needed to verify computational models which are the main tool for research on this type of flow.  The proposed equipment will be configured into a measurement system that will determine fluid velocity by tracking tagged fluorescent molecules.  The proposed system will have many unique features that will form a powerful experimental tool.  These features include, all optical access from one view port, the ability to write a fine, unique pattern of dots into the flow increasing measurement resolution and complete computer control of the system.  This style of measure cannot be purchased commercially and all equipment has been specifically specified for the unique conditions found in micro-channel flows.