Using quantitative PCR as part of a multilevel approach to neuroscience

使用定量 PCR 作为神经科学多层次方法的一部分

• 批准号: 359623-2008
• 负责人: PerrotSinal, Tara
• 金额: $ 3.49万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365602
• 关键词: Using; quantitative; PCR; part; multilevel

The requested funds will be used to purchase a system that will allow us to measure levels of genes in brain tissue generated from animal models.  The system will be used by 5 researchers, each of whom is interested in a different aspect of brain function.  For example, this system will be used to measure levels of genes involved in the function of brain cell communication and also used to measure levels of genes potentially involved in the cognitive deficits associated with Alzheimer's disease.  These examples serve to illustrate the broad range of uses of this system and the multi-level approach to neuroscience that occurs in the Psychology department at Dalhousie University.  Other uses for this equipment will include examining how stress affects levels of genes important for brain function, investigating the importance of structural genes for development of brain regions important for vision, and examining genes involved in development of the auditory system in songbirds.  In addition to providing a vital tool for the above-mentioned research projects, this system will be used to train highly qualified personnel (undergraduate, graduate students, postdoctoral fellows, technicians) from each of the co-applicant's labs.

所请求的资金将用于购买一个系统，该系统将使我们能够测量动物模型产生的脑组织中的基因水平。  该系统将由 5 名研究人员使用，他们每人都对大脑功能的不同方面感兴趣。  例如，该系统将用于测量涉及脑细胞通讯功能的基因水平，还用于测量可能涉及与阿尔茨海默病相关的认知缺陷的基因水平。  这些例子说明了该系统的广泛用途以及达尔豪斯大学心理学系的神经科学多层次方法。  该设备的其他用途包括检查压力如何影响对大脑功能重要的基因水平，研究结构基因对视觉重要大脑区域发育的重要性，以及检查与鸣禽听觉系统发育有关的基因。  除了为上述研究项目提供重要工具外，该系统还将用于培养各共同申请人实验室的高素质人才（本科生、研究生、博士后、技术人员）。