Spectrophotometric/fluorometric plate reader for metabolic assays

用于代谢测定的分光光度/荧光读板仪

• 批准号: 359202-2008
• 负责人: Dyck, David
• 金额: $ 3.07万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365877
• 关键词: Spectrophotometric; fluorometric; plate; reader; metabolic

This application is for a spectrophotometric/fluorometric plate reader to be used for muscle and blood metabolite assays. The applicants for this RTI grant are Drs. David J. Dyck (principal applicant) and Lawrence L. Spriet (co-applicant). Both Drs. Dyck and Spriet examine the regulation of carbohydrate and lipid metabolism in mammalian skeletal muscle, and each currently hold NSERC Discovery grants.  In addition, Dr. Dyck holds a CIHR grant. The main objective of Dr. Dyck's research program is to understand the basic regulation of fatty acid (FA) metabolism in skeletal muscle. Specifically, these objectives include the role of i) Ca2+, energy charge and AMP-activated protein kinase; ii) hormones including leptin, adiponectin, resistin, IL-6, TNFa; and iii) altered FA metabolism in obesity/diabetes. Dr. Spriet examines the regulaton of fat and carbohydrate metabolism in human skeletal muscle, with emphasis on both exercise and disease (obesity) situations. Collectively, projects from these investigators require the extensive analyses of muscle and blood metabolites related to carbohydrate and lipid metabolism - these are core measurements in all of our studies.

该应用程序适用于用于肌肉和血液代谢物测定的分光光度/荧光板读数器。此项 RTI 补助金的申请人是 Drs。 David J. Dyck（主申请人）和 Lawrence L. Spriet（共同申请人）。两位博士。 Dyck 和 Spriet 研究了哺乳动物骨骼肌中碳水化合物和脂质代谢的调节，目前两人都持有 NSERC 发现资助。  此外，Dyck 博士还获得 CIHR 资助。 Dyck 博士研究项目的主要目标是了解骨骼肌中脂肪酸 (FA) 代谢的基本调节。具体来说，这些目标包括 i) Ca2+、能量电荷和 AMP 激活蛋白激酶的作用； ii) 激素，包括瘦素、脂联素、抵抗素、IL-6、TNFa； iii) 改变肥胖/糖尿病中的 FA 代谢。 Spriet 博士研究人体骨骼肌中脂肪和碳水化合物代谢的调节，重点关注运动和疾病（肥胖）情况。总的来说，这些研究人员的项目需要对与碳水化合物和脂质代谢相关的肌肉和血液代谢物进行广泛分析——这些是我们所有研究的核心测量值。