Scintillation counter for metabolic studies.

用于代谢研究的闪烁计数器。

• 批准号: RTI-2018-00259
• 负责人: Dyck, David
• 金额: $ 6.22万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642187
• 关键词: Scintillation; counter; metabolic; studies.

This application is for a liquid scintillation counter to be used for muscle, adipose and liver metabolic assays. The purpose of the scintillation counter is to detect small levels of radiation given off by radiolabelled substrates used in our assays to assess the uptake and metabolism of glucose (sugar), fatty acids, choline (which is incorporated into membranes), etc. These methods are fundamental to the NSERC-funded research programs of the 3 applicants, Dr. Dyck (principal applicant) and Drs. Bakovic and Spriet (co-applicants), as well as an additional user, Dr. Holloway. The overall objective of Dr. Dyck's research program is to understand the major sites of regulation (transport, storage, oxidation) of skeletal muscle glucose and fatty acid metabolism, and how this affects insulin response. Dr. Bakovic's laboratory focuses on regulation of membrane transport mechanisms and phospholipid homeostasis. Dr. Spriet’s research uses animal and human models to study the regulation of the pathways that metabolize carbohydrate and lipid as fuel to produce energy in skeletal muscle and heart. Dr. Holloway's research program is primarily focused on understanding the regulation of fatty acid metabolism in the context of health and disease. His experimental approaches involve using exercise and diet as modalities to alter fatty acid transport across the plasma and mitochondrial membranes. All of the above research programs require the use of small amount of radiolabelled substrates and metabolites to study functional processes such as the uptake and metabolism of glucose, fatty acids, choline, etc. These measurements are a key technique in the training of our HQP and an essential measurement in many of our studies. Collectively, over 40% of our journal publications within the last 6 years of all applicants utilized radioisotopes and therefore a liquid scintillation counter. The bulk of our personnel are PhD and MSc trainees whose productivity and subsequent competitiveness for scholarships, fellowships and future employment hinges on their access to a scintillation counter to perform these necessary functional metabolic measurements. Simply put, without a scintillation counter to detect the various isotopes used in our studies, this critical analyses cannot be carried out. There is currently one scintillation counter in our department, now over 20 years old, and in fact, it is the only one in our entire building. Purchasing a scintillation counter is essential for the continued research of the applicants, and the training and productivity of our HQP.

该应用是用于肌肉、脂肪和肝脏代谢分析的液体闪烁计数器。闪烁计数器的目的是检测我们分析中使用的放射性标记底物发出的少量辐射，以评估葡萄糖(糖)、脂肪酸、胆碱(被结合到膜中)等的吸收和代谢。这些方法是NSERC资助的三位申请者Dyck博士(主要申请者)、Bakovic博士和Spriet博士(共同申请者)以及另一位使用者Dr.Holloway的研究计划的基础。戴克博士研究计划的总体目标是了解骨骼肌葡萄糖和脂肪酸代谢的主要调节部位(运输、储存、氧化)，以及这如何影响胰岛素反应。巴科维奇博士的实验室专注于膜转运机制和磷脂动态平衡的调节。斯普里特博士的研究使用动物和人体模型来研究碳水化合物和脂肪代谢途径的调节，这些途径将碳水化合物和脂肪作为燃料在骨骼肌和心脏产生能量。霍洛韦博士的研究项目主要集中在从健康和疾病的角度理解脂肪酸代谢的调节。他的实验方法包括使用运动和饮食作为改变脂肪酸跨血浆和线粒体膜运输的方式。上述所有研究计划都需要使用少量的放射性标记底物和代谢物来研究功能过程，如葡萄糖、脂肪酸、胆碱等的吸收和代谢。这些测量是我们HQP培训中的关键技术，也是我们许多研究中的基本测量。总的来说，在所有申请者的过去6年中，我们超过40%的期刊出版物使用了放射性同位素，因此使用了液体闪烁计数器。我们的大部分人员是博士生和理科实习生，他们的生产力和随后获得奖学金、奖学金和未来就业的竞争力取决于他们能否使用闪烁计数器来执行这些必要的功能代谢测量。简而言之，如果没有闪烁计数器来检测我们研究中使用的各种同位素，就不能进行这种关键的分析。目前我们部门有一个闪烁计数器，已经有20多年的历史了，实际上，它是我们整个建筑中唯一的一个。购买闪烁计数器对于申请者的继续研究以及我们HQP的培训和生产力是必不可少的。