Scintillation counter for metabolic studies.

此应用程序是用于肌肉，脂肪和肝脏代谢测定的液体闪烁计数器。闪烁计数器的目的是检测我们用于评估葡萄糖（糖）、脂肪酸、胆碱（被纳入膜）等的摄取和代谢的放射性标记底物发出的小水平辐射。这些方法是nserc资助的3位申请人，Dyck博士（主申请人）和dr。Bakovic和sprieet（共同申请者），以及另外一位用户Holloway博士。Dyck博士研究项目的总体目标是了解骨骼肌葡萄糖和脂肪酸代谢的主要调控位点（运输、储存、氧化），以及这如何影响胰岛素反应。Bakovic博士的实验室专注于膜运输机制和磷脂稳态的调节。Spriet博士的研究使用动物和人类模型来研究代谢碳水化合物和脂质作为燃料以在骨骼肌和心脏中产生能量的途径的调节。Holloway博士的研究项目主要集中在了解健康和疾病背景下脂肪酸代谢的调节。他的实验方法包括用运动和饮食作为改变脂肪酸在血浆和线粒体膜上运输的方式。上述所有研究项目都需要使用少量放射性标记的底物和代谢物来研究功能过程，如葡萄糖、脂肪酸、胆碱等的摄取和代谢。这些测量是HQP培训的关键技术，也是我们许多研究的基本测量。总的来说，在过去6年中，我们所有申请人的期刊出版物中超过40%使用了放射性同位素，因此使用了液体闪烁计数器。我们的大部分员工都是博士和硕士学员，他们的生产力以及随后在奖学金、奖学金和未来就业方面的竞争力取决于他们是否能够使用闪烁计数器来执行这些必要的功能性代谢测量。简单地说，如果没有闪烁计数器来检测我们研究中使用的各种同位素，就无法进行这种关键的分析。目前我们部门有一个闪烁计数器，已经有20多年的历史了，事实上，它是我们整个大楼里唯一的一个。购买闪烁计数器对于申请人的持续研究，以及我们HQP的培训和生产力至关重要。

This application is for a liquid scintillation counter to be used for muscle, adipose and liver metabolic assays. The purpose of the scintillation counter is to detect small levels of radiation given off by radiolabelled substrates used in our assays to assess the uptake and metabolism of glucose (sugar), fatty acids, choline (which is incorporated into membranes), etc. These methods are fundamental to the NSERC-funded research programs of the 3 applicants, Dr. Dyck (principal applicant) and Drs. Bakovic and Spriet (co-applicants), as well as an additional user, Dr. Holloway. The overall objective of Dr. Dyck's research program is to understand the major sites of regulation (transport, storage, oxidation) of skeletal muscle glucose and fatty acid metabolism, and how this affects insulin response. Dr. Bakovic's laboratory focuses on regulation of membrane transport mechanisms and phospholipid homeostasis. Dr. Spriet’s research uses animal and human models to study the regulation of the pathways that metabolize carbohydrate and lipid as fuel to produce energy in skeletal muscle and heart. Dr. Holloway's research program is primarily focused on understanding the regulation of fatty acid metabolism in the context of health and disease. His experimental approaches involve using exercise and diet as modalities to alter fatty acid transport across the plasma and mitochondrial membranes. All of the above research programs require the use of small amount of radiolabelled substrates and metabolites to study functional processes such as the uptake and metabolism of glucose, fatty acids, choline, etc. These measurements are a key technique in the training of our HQP and an essential measurement in many of our studies. Collectively, over 40% of our journal publications within the last 6 years of all applicants utilized radioisotopes and therefore a liquid scintillation counter. The bulk of our personnel are PhD and MSc trainees whose productivity and subsequent competitiveness for scholarships, fellowships and future employment hinges on their access to a scintillation counter to perform these necessary functional metabolic measurements. Simply put, without a scintillation counter to detect the various isotopes used in our studies, this critical analyses cannot be carried out. There is currently one scintillation counter in our department, now over 20 years old, and in fact, it is the only one in our entire building. Purchasing a scintillation counter is essential for the continued research of the applicants, and the training and productivity of our HQP.