Protecting Canada's drinking water: Developing real-time early-warning biomonitoring technology

保护加拿大的饮用水：开发实时预警生物监测技术

• 批准号: 351062-2007
• 负责人: McCarthy, Lynda
• 金额: $ 9.07万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365990
• 关键词: Protecting; Canada; drinking; water; Developing

This proposal addresses the necessity of developing an early-warning strategy to detect potential threats todrinking water sources. Natural bodies of water that ultimately constitute some of Canada's drinking watersupply can contain a variety of pollutants such as heavy metals, PAHS, halogenated organic molecules,pharmaceuticals, pathogens, etc. While the associated health risks of some of these components are alreadyknown, there remains an alarming lack of research in many other areas. Our proposal will develop aninnovative approach to assessing the potential toxicity of waterborne contaminants before these deleteriousconstituents reach drinking-water facilities. We will use a holistic, multi organism-based system to measurestress reactions in the aquatic community and will monitor these stress responses in real-time. We will alsodevelop ecotoxicity models based on dose-responses of individual organisms to specific classes of stressors(chemical and biological). These models will aid in the interpretation of the data and will provide informationfor water-utilities managers about the nature of the stressor. Also, this knowledge will be useful in strategies tominimize risk to end-users. The ultimate goal of our proposal is the development and implementation of anearly-warning system in real-time for drinking-water facilities that would detect chemical contaminants andpathogens using biomonitoring organisms. In order to achieve this, our project will: 1) measure responses inaquatic plants and invertebrates to chemical stressors at environmentally-relevant concentrations and topathogens; 2) develop a microarray-based test that can be used to directly detect pathogenic organisms; 3) buildand test a flow-through system in a drinking-water facility for stress-response determinations in real-time; 4)develop methods of stereotyped responses for the whole suite of biomonitoring organisms to chemicalcontaminants and pathogens. These models will be used to predict the nature of contamination when responsesare observed among the organisms. These models will ultimately form the basis of the early-warning system tomonitor potential toxicity of chemical and biological agents in real-time at drinking-water facilities.

该提案提出了制定早期预警战略以发现对饮用水源的潜在威胁的必要性。最终构成加拿大一些饮用水供应的天然水体可能含有各种污染物，如重金属、多环芳烃、卤化有机分子、药物、病原体等。虽然其中一些成分的相关健康风险已经为人所知，但在许多其他领域的研究仍然令人震惊地缺乏。我们的提案将开发一种创新的方法，在这些有害成分到达饮用水设施之前评估水生污染物的潜在毒性。我们将使用一个整体的、基于多生物的系统来测量水生群落的应激反应，并将实时监测这些应激反应。我们还将开发基于个体生物对特定种类的压力源（化学和生物）的剂量反应的生态毒性模型。这些模型将有助于对数据的解释，并将为水务管理人员提供有关压力源性质的信息。此外，这些知识将有助于将最终用户的风险降至最低。我们提案的最终目标是为饮用水设施开发和实施一个实时预警系统，该系统将使用生物监测生物检测化学污染物和病原体。为了实现这一目标，我们的项目将：1)测量水生植物和无脊椎动物对环境相关浓度和病原体的化学应激源的反应；2)开发一种基于微阵列的检测方法，可用于直接检测病原生物；3)在饮用水设施中建立并测试一个流过系统，以实时确定应力响应；4)发展一整套生物监测生物对化学污染物和病原体的定型反应方法。当在生物体中观察到反应时，这些模型将用于预测污染的性质。这些模型最终将构成预警系统的基础，以实时监测饮用水设施中化学和生物制剂的潜在毒性。