The role of prostaglandins in the control of intestinal motility in physiological and experimental inflammatory states.

前列腺素在生理和实验炎症状态下肠道运动控制中的作用。

• 批准号: nhmrc : 102104
• 负责人: Prof Marcello Costa
• 金额: $ 21.08万
• 依托单位: Flinders University
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/role-prostaglandins-control-inflammatory-states/76855
• 关键词: role; prostaglandins; control; intestinal; motility

Many intestinal disorders are due to inflammations of unknown origin, associated with pain, diarrhoea or constipation. How this occurs is not known. The movements of the intestine are due to the contractions and relaxations of the muscular wall, which are controlled by a network of nerve cells, a kind of a brain in the gut. In inflammatory conditions, a multitude of chemical substances are produced by the sick gut. Among these substances are the prostaglandins that are responsible for increasing pain from inflamed parts. However, the gut makes prostaglandins even when there is no inflammation, although it is not clear what do these substances do in the normal intestine. During disease, prostaglandins are made in much larger amounts. If we can establish what they do normally we may be able to establish how they work in disease. Therefore our projects is in two parts. First, we will investigate how prostaglandins normally affect the working of the nerves and muscle controlling intestinal movement. In the second part we will reveal the role of these substances during mild inflammation induced in some laboratory animals to mimic human diseases. All experiments will be carried out on intestines removed from these experimental animals after they are killed humanely. This enables us to study how the experimentally induced diseases affect gut function, especially movement. We will use a method, that has recently been developed in our laboratory, to transform video recordings of gut movements into computer-generated maps. From these pictures, we can see patterns of movement that are too subtle to detect by just watching the videos. We will end our project by establishing if and when prostaglandins are responsible for producing the abnormal intestinal movements seen in disease. This will give clinicians a better basis to develop new drugs against gut disorders.

许多肠道疾病是由不明原因的炎症引起的，伴有疼痛、腹泻或便秘。这是如何发生的尚不清楚。肠道的运动是由于肌肉壁的收缩和松弛而产生的，而肌肉壁的收缩和松弛是由神经细胞网络（肠道中的一种大脑）控制的。在炎症条件下，患病的肠道会产生多种化学物质。这些物质中的前列腺素会加剧发炎部位的疼痛。然而，即使没有炎症，肠道也会产生前列腺素，尽管尚不清楚这些物质在正常肠道中的作用。在疾病期间，前列腺素的产生量要大得多。如果我们能够确定它们在正常情况下的作用，我们也许就能确定它们在疾病中的作用。因此我们的项目分为两部分。首先，我们将研究前列腺素通常如何影响控制肠道运动的神经和肌肉的工作。在第二部分中，我们将揭示这些物质在一些模拟人类疾病的实验动物中诱导轻度炎症过程中的作用。所有实验都将在这些实验动物被人道处死后取出的肠子上进行。这使我们能够研究实验诱发的疾病如何影响肠道功能，尤其是运动。我们将使用我们实验室最近开发的一种方法，将肠道运动的视频记录转换为计算机生成的地图。从这些图片中，我们可以看到非常微妙的运动模式，仅通过观看视频无法察觉。我们将通过确定前列腺素是否以及何时负责产生疾病中所见的异常肠道运动来结束我们的项目。这将为临床医生开发针对肠道疾病的新药奠定更好的基础。