Interaction between trichoderma aggressivum and agaricus bisporus

前言：同一物种或不同物种的真菌细胞之间的对抗会导致多种事件之一，包括没有明显的相互作用、回避、生长抑制、一种或两种真菌的死亡，或者细胞融合(寄生的、营养的或有性融合)。早期的细胞识别事件是通过产生和接收可扩散的信号或通过表面配体和受体介导的细胞对细胞的直接接触来调节的。这项拟议的研究将调查侵袭木霉菌(蘑菇绿霉病的病原体)和双孢菇(商品蘑菇)相互作用过程中的早期分子事件。这项研究的具体短期目标是：(1)确定不同品系的商品蘑菇在抑制方面的差异是否由于N-乙酰氨基葡萄糖苷酶产生的变化；(2)确定抑制方面的差异是否与木霉毒素的不同代谢有关；(3)确定双孢霉产生的信号(S)的性质，该信号导致侵袭性木霉增加水解酶的产生；以及(4)确定侵袭性木霉产生的几丁质酶、葡聚糖酶和蛋白酶是否在绿霉病的发展中发挥任何作用。意义：长期目标包括定义信号产生、接收和转导的遗传控制，以及确定这些机制在其他真菌-真菌相互作用中的普遍性。这种性质的工作将使人们深入了解不同的领域，如寄生真菌在动植物组织中的识别、附着和疾病发展，以及将真菌用作其他真菌的生防剂。这些研究也将为了解侵袭性毛滴虫和双孢霉之间的疾病关系提供依据。一个具有重大经济意义的长期目标是制定程序，防止或破坏这种关系，从而导致疾病控制。

Introduction: Confrontation between fungal cells of the same species or different species leads to one of a variety of events including no apparent interaction, avoidance, growth inhibition, death of one or both fungi, or cell fusion (parasitic, vegetative or sexual fusion). Early cellular recognition events are mediated by production and reception of diffusible signals or through direct cell to cell contact mediated by surface ligands and receptors. The proposed research will investigate early molecular events during the interaction between Trichoderma aggressivum, the causative agent of mushroom green mould disease, and Agaricus bisporus, the commercial mushroom. Specific short-term goals of the research are: (1) to determine if differences in inhibition shown by various strains of the commercial mushroom are due to altered N-acetylglucosaminidase production; (2) to determine if differences in inhibition are associated with differential metabolism of Trichoderma toxins; (3) to determine the nature of the signal(s) produced by A. bisporus which lead to increased production of hydrolytic enzymes by T. aggressivum; and (4) to determine if chitinases, glucanases and proteinases produced by T. aggressivum play any role in green mold disease progression. Significance: Longer-term goals include definition of the genetic control of signal production, reception and transduction and determination of the universality of these mechanisms within other fungus-fungus interactions. Work of this nature will give insight into diverse areas such as recognition, attachment and disease progression in plant and animal tissues by parasitic fungi and the use of fungi as biocontrol agents of other fungi. These studies will also provide a basis for understanding the disease relationship between T. aggressivum and A. bisporus. An economically significant long-term goal is to develop procedures that will prevent or disrupt the relationship and thereby result in disease control.