Interaction between trichoderma aggressivum and agaricus bisporus

简介：相同物种或不同物种的真菌细胞之间的对抗导致多种事件之一，包括没有明显的相互作用、回避、生长抑制、一种或两种真菌的死亡或细胞融合（寄生、营养或有性融合）。早期的细胞识别事件是由可扩散信号的产生和接收介导的，或通过由表面配体和受体介导的直接细胞与细胞接触介导的。本研究将探讨香菇绿色病害病原侵袭木霉与商品蘑菇双孢蘑菇相互作用过程中的早期分子事件。研究的具体短期目标是：（1）确定不同商业蘑菇菌株所显示的抑制差异是否是由于N-乙酰氨基葡萄糖苷酶产生的改变;（2）确定抑制差异是否与木霉毒素的差异代谢有关;（3）确定A. bisporus，导致T.（4）测定T.在绿色霉菌疾病进展中起任何作用。重要性：长期目标包括确定信号产生、接收和转导的遗传控制，并确定这些机制在其他真菌-真菌相互作用中的普遍性。这种性质的工作将使人们深入了解不同的领域，如寄生真菌在植物和动物组织中的识别、附着和疾病进展，以及真菌作为其他真菌生物防治剂的用途。这些研究也将为了解T. aggressivum和A.双孢菌。一个具有经济意义的长期目标是开发将预防或破坏这种关系的程序，从而控制疾病。

Introduction: Confrontation between fungal cells of the same species or different species leads to one of a variety of events including no apparent interaction, avoidance, growth inhibition, death of one or both fungi, or cell fusion (parasitic, vegetative or sexual fusion). Early cellular recognition events are mediated by production and reception of diffusible signals or through direct cell to cell contact mediated by surface ligands and receptors. The proposed research will investigate early molecular events during the interaction between Trichoderma aggressivum, the causative agent of mushroom green mould disease, and Agaricus bisporus, the commercial mushroom. Specific short-term goals of the research are: (1) to determine if differences in inhibition shown by various strains of the commercial mushroom are due to altered N-acetylglucosaminidase production; (2) to determine if differences in inhibition are associated with differential metabolism of Trichoderma toxins; (3) to determine the nature of the signal(s) produced by A. bisporus which lead to increased production of hydrolytic enzymes by T. aggressivum; and (4) to determine if chitinases, glucanases and proteinases produced by T. aggressivum play any role in green mold disease progression. Significance: Longer-term goals include definition of the genetic control of signal production, reception and transduction and determination of the universality of these mechanisms within other fungus-fungus interactions. Work of this nature will give insight into diverse areas such as recognition, attachment and disease progression in plant and animal tissues by parasitic fungi and the use of fungi as biocontrol agents of other fungi. These studies will also provide a basis for understanding the disease relationship between T. aggressivum and A. bisporus. An economically significant long-term goal is to develop procedures that will prevent or disrupt the relationship and thereby result in disease control.