Interaction between trichoderma aggressivum and agaricus bisporus

同一种或不同种真菌细胞之间的对抗会导致多种事件中的一种，包括无明显相互作用、回避、生长抑制、一种或两种真菌死亡或细胞融合（寄生、营养或性融合）。早期的细胞识别事件是通过产生和接收扩散性信号或通过由表面配体和受体介导的细胞间直接接触介导的。拟议的研究将调查侵染木霉（蘑菇绿霉病的病原体）和双孢蘑菇（商品蘑菇）相互作用过程中的早期分子事件。具体的短期研究目标是：(1)确定不同菌种对商品蘑菇的抑制差异是否由于n -乙酰氨基葡萄糖苷酶产生的改变；(2)确定抑制差异是否与木霉毒素代谢差异有关；(3)确定双孢芽孢杆菌产生的导致侵略性芽孢杆菌水解酶产量增加的信号的性质；(4)确定侵袭性托菌产生的几丁质酶、葡聚糖酶和蛋白酶是否在绿霉病的进展中起作用。意义：长期目标包括定义信号产生、接收和转导的遗传控制，并确定这些机制在其他真菌-真菌相互作用中的普遍性。这种性质的工作将使人们深入了解寄生真菌在植物和动物组织中的识别、附着和疾病进展，以及真菌作为其他真菌的生物防治剂的使用。这些研究也将为了解侵染性芽孢杆菌与双孢单胞菌之间的疾病关系提供依据。一个具有经济意义的长期目标是开发能够预防或破坏这种关系的程序，从而实现疾病控制。

Introduction: Confrontation between fungal cells of the same species or different species leads to one of a variety of events including no apparent interaction, avoidance, growth inhibition, death of one or both fungi, or cell fusion (parasitic, vegetative or sexual fusion). Early cellular recognition events are mediated by production and reception of diffusible signals or through direct cell to cell contact mediated by surface ligands and receptors. The proposed research will investigate early molecular events during the interaction between Trichoderma aggressivum, the causative agent of mushroom green mould disease, and Agaricus bisporus, the commercial mushroom. Specific short-term goals of the research are: (1) to determine if differences in inhibition shown by various strains of the commercial mushroom are due to altered N-acetylglucosaminidase production; (2) to determine if differences in inhibition are associated with differential metabolism of Trichoderma toxins; (3) to determine the nature of the signal(s) produced by A. bisporus which lead to increased production of hydrolytic enzymes by T. aggressivum; and (4) to determine if chitinases, glucanases and proteinases produced by T. aggressivum play any role in green mold disease progression. Significance: Longer-term goals include definition of the genetic control of signal production, reception and transduction and determination of the universality of these mechanisms within other fungus-fungus interactions. Work of this nature will give insight into diverse areas such as recognition, attachment and disease progression in plant and animal tissues by parasitic fungi and the use of fungi as biocontrol agents of other fungi. These studies will also provide a basis for understanding the disease relationship between T. aggressivum and A. bisporus. An economically significant long-term goal is to develop procedures that will prevent or disrupt the relationship and thereby result in disease control.