Impact of wastewater particles on ultraviolet and chlorine disinfection and public health

废水颗粒物对紫外线和氯消毒及公共卫生的影响

• 批准号: 326978-2006
• 负责人: Örmeci, Banu
• 金额: $ 1.89万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=397507
• 关键词: Impact; wastewater; particles; ultraviolet; chlorine

Protecting public health is a primary objective of wastewater treatment facilities, and disinfection is the most important process to control pathogens. Previous research has documented that presence of wastewater particulates affect the disinfection process, and coliform bacteria, the most commonly used indicator microorganism, associated with particles survive typical and much higher doses of disinfection applied in treatment plants. This is troublesome because the survival of particle-associated coliform through a disinfection process may indicate the survival of other more resistant health related microorganisms and may pose a threat to public health. The main objective of this research is to determine to what extent microorganisms are associated with particles, how they are distributed within the floc structure, and how their spatial distribution influences their response to disinfection by chlorine and UV radiation. Another objective is to determine how far into the floc structure UV and chlorine can penetrate and be able to inactivate particle-associated microorganisms. The research will employ a combination of molecular methods, including fluorescence in situ hybridization (FISH), indirect immunofluorescence, and ethidium monoazide (EMA) staining coupled with laser cytometry to provide information on the number, distribution, identity, and viability of particle-associated microorganisms after UV and chlorine disinfection. The study will improve the understanding of the fate and survival of pathogens through a disinfection process in a wastewater treatment plant. The acquired knowledge will benefit regulators, utilities, and consultants, and will help to improve the performance and design of existing and future disinfection processes. This knowledge is useful to provide wastewater facility operators with improved process controls, for consistency in meeting disinfection targets, and is the first step in protecting public health.

保护公众健康是污水处理设施的首要目标，而消毒是控制病原体的最重要环节。以前的研究已经证明，废水颗粒物的存在会影响消毒过程，而与颗粒物相关的最常用的指示微生物--大肠菌群--在处理厂应用的典型消毒剂量和更高剂量下仍能存活。这很麻烦，因为与颗粒相关的大肠菌群通过消毒过程存活可能意味着其他更具抵抗力的健康相关微生物的存活，并可能对公众健康构成威胁。这项研究的主要目的是确定微生物与颗粒的关联程度，它们在絮体结构中的分布情况，以及它们的空间分布如何影响它们对氯和紫外线辐射消毒的反应。另一个目标是确定紫外线和氯能在多大程度上渗透到絮体结构中并能够灭活与颗粒相关的微生物。这项研究将使用分子方法的组合，包括荧光原位杂交(FISH)、间接免疫荧光、单叠氮乙锭(EMA)染色和激光细胞仪，以提供紫外线和氯消毒后颗粒相关微生物的数量、分布、身份和生存能力的信息。这项研究将通过污水处理厂的消毒过程提高对病原体命运和生存的理解。所获得的知识将使监管机构、公用事业公司和顾问受益，并将有助于改进现有和未来消毒工艺的性能和设计。这一知识有助于为废水设施操作员提供改进的过程控制，确保达到消毒目标的一致性，是保护公众健康的第一步。