Molecular and functional analysis of nuclear membrane localized GnRH-RI

核膜定位 GnRH-RI 的分子和功能分析

• 批准号: 327334-2006
• 负责人: Babwah, Andy
• 金额: $ 2.77万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=397722
• 关键词: Molecular; functional; analysis; nuclear; membrane

The gonadotropin releasing hormone receptor is a protein molecule that is found in the outer membrane of certain cells in humans and animals including the chimpanzee, chicken and fish.  This receptor recognizes specific physiological stimuli and directs the cell in the body on how to respond accordingly.  Some of these stimuli arise during reproductive maturation, pregnancy and even infections and results in the cell producing very important proteins.  To date we have a very poor understanding on how these receptor proteins carry out these functions and it is important that we do.  This understanding will facilitate the development of more successful strategies to maintain the health and reproductive potential of livestock as well as understand the underlying mechanisms of some human conditions related to delayed or diminished reproductive development.  We have recently discovered that in addition to residing in the outer cell membrane the gonadotropin releasing hormone receptor also resides within the cell on the nucleus.  We believe it does so in order to respond much more quickly and efficiently to the physiological stimuli and this may represent a recent evolutionary advancement among receptor proteins.  We are currently testing these ideas which challenge the currently held views on how this class of receptor functions in the cell.

促性腺激素释放激素受体是一种蛋白质分子，存在于人类和包括黑猩猩、鸡和鱼在内的动物的某些细胞的外膜中。这种受体识别特定的生理刺激，并指导体内的细胞如何做出相应的反应。这些刺激在生殖成熟、怀孕甚至感染期间产生，导致细胞产生非常重要的蛋白质。到目前为止，我们对这些受体蛋白是如何发挥这些功能的了解甚少，而了解这些功能是很重要的。这种了解将有助于制定更成功的战略，以维持牲畜的健康和生殖潜力，并了解与生殖发育延迟或减少有关的某些人类状况的潜在机制。我们最近发现，除了存在于细胞膜外，促性腺激素释放激素受体也存在于细胞核上的细胞内。我们认为，它这样做是为了更快、更有效地对生理刺激作出反应，这可能代表了受体蛋白最近的进化进展。我们目前正在测试这些想法，这些想法挑战了目前关于这类受体如何在细胞中发挥作用的观点。