Molecular and functional analysis of nuclear membrane localized GnRH-RI

核膜定位 GnRH-RI 的分子和功能分析

• 批准号: 327334-2006
• 负责人: Babwah, Andy
• 金额: $ 2.77万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=397722
• 关键词: Molecular; functional; analysis; nuclear; membrane

The gonadotropin releasing hormone receptor is a protein molecule that is found in the outer membrane of certain cells in humans and animals including the chimpanzee, chicken and fish.  This receptor recognizes specific physiological stimuli and directs the cell in the body on how to respond accordingly.  Some of these stimuli arise during reproductive maturation, pregnancy and even infections and results in the cell producing very important proteins.  To date we have a very poor understanding on how these receptor proteins carry out these functions and it is important that we do.  This understanding will facilitate the development of more successful strategies to maintain the health and reproductive potential of livestock as well as understand the underlying mechanisms of some human conditions related to delayed or diminished reproductive development.  We have recently discovered that in addition to residing in the outer cell membrane the gonadotropin releasing hormone receptor also resides within the cell on the nucleus.  We believe it does so in order to respond much more quickly and efficiently to the physiological stimuli and this may represent a recent evolutionary advancement among receptor proteins.  We are currently testing these ideas which challenge the currently held views on how this class of receptor functions in the cell.

促性腺激素释放的马酮受体是一种蛋白质分子，在人类和动物的某些细胞的外膜中发现，包括黑猩猩，鸡肉和鱼。该受体识别特定的物理刺激，并指导体内的细胞如何相应地反应。这些刺激中的一些是在生殖成熟，妊娠甚至感染过程中引起的，并导致细胞产生非常重要的蛋白质。迄今为止，我们对这些受体蛋白如何执行这些功能的了解非常糟糕，这一点很重要。这种理解将支持开发更成功的策略，以维持牲畜的健康和生殖潜力，并了解与延迟或减少生殖发展有关的某些人类状况的潜在机制。我们最近发现，除了居住在外部细胞膜中，促性腺激素释放的马酮受体还位于核us的细胞内。我们认为，这样做是为了更快，更有效地对物理刺激做出反应，这可能代表了受体蛋白质中最近的进化进步。我们目前正在测试这些想法，这些想法挑战了当前对这类受体在细胞中如何功能的看法。