Regulation of claudin expression in chick embryos

所有脊椎动物在外表上都是两侧对称的，但在内部器官在体内的位置上有大量的不对称。我们感兴趣的是理解一套“胚胎指令”，这些指令指示单个器官特定地定位于胚胎的左侧或右侧。有趣的是，这种模式在进化上是保守的。目前，我们正在研究这些机制，这些机制负责这一过程的初始步骤，并将两侧对称的胚胎转化为具有明显左右不对称的胚胎。我们正在研究的分子之一是Claudin-1，这是一种紧密连接蛋白，有助于调节分子在细胞层之间的运动，并保持分子在细胞表面的适当分布。我们已经证明，在胚胎发生过程中，Claudin-1的不适当表达改变了胚胎的正常不对称发育。因此，我们假设Claudin-1的动态表达模式必须是暂时的和空间上调节的，才能实现正常的胚胎发育。

All vertebrates are bilaterally symmetrical on the outside but internally they have a large number of asymmetries with respect to how the internal organs are positioned within the body. We are interested in understanding the set of "embryonic instructions" that direct the individual organs to become specifically localized to the left or right sides of the embryo. Interestingly, this pattern has been evolutionarily conserved. Currently, we are investigating those mechanisms that are responsible for the initial steps of this process and convert a bilaterally symmetrical embryo into an embryo with distinct left and right asymmetries. One of the molecules that we are studying is Claudin-1, a tight junction protein that helps to regulate the movement of molecules between cell layers and to maintain the proper distribution of molecules on the cell surface. We have shown that the inappropriate expression of Claudin-1 during embryogenesis alters the normal asymmetric development of the embryo. Therefore, we hypothesize that the dynamic expression pattern of Claudin-1 must be temporarily and spatially regulated for normal embryogenesis.