Ultra-centrifuge - Steacie Supplement

目前，心理学系没有超速离心机。离我们最近的是生物系(四层楼下，大楼的隔壁)。他们非常慷慨地允许我们使用它，然而，我们发现，随着我们开始检查有助于重新巩固的突触蛋白，我们使用超速离心机的频率正在变得越来越高。我们认为这一领域的研究是我们未来5-10年的重点。鉴于世界上很少有实验室专注于杏仁核这一问题，我们现在发表一些将杏仁核受体贩运与行为变化联系起来的研究是非常合适的。这将使我们处于这一领域的领先地位。很少有小组能够将行为与突触蛋白联系起来，我们有PKMzeta的数据表明该项目是可行的。超离心机是实现这一目标的核心。这是唯一一种可以定量评估突触后密度中蛋白质的方法，相对较少受到其他细胞隔间的污染。

Currently the Dept of Psychology does not have an ultra-centrifuge. The closest one to us is in the Biology Department (4 floors down and the adjacent wing of the building). They have been very generous in letting us use it, however, we are finding that our use of the ultra-centrifuge is becoming more frequent as we begin to examine synaptic proteins that contribute to reconsolidation. We feel this area of research is the next 5-10 yrs of focus for us. Given that few labs in the world are focused on this issue amygdala, we are in a very opportune moment to publish some studies linking receptor trafficking in the amygdala with changes in behavior. This would place us at the lead of this portion of the field. Few groups have been able to make the link from behavior to synaptic proteins and we have data with PKMzeta indicating that the project is feasible. The ultra-centrifuge is central to make that happen. It is the only method to allow a quantitative evaluation of proteins in the post-synaptic density, with relatively little contamination from other cellular compartments.