Cell survival rate in automated cell injection and development of automated injection system

自动细胞注射中的细胞存活率和自动注射系统的开发

• 批准号: 374690-2009
• 负责人: Mills, James
• 金额: $ 5.01万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=411989
• 关键词: Cell; survival; rate; automated; cell

Recent advances in biological fields have heightened the interest and need for reliable techniques which may be carried out on a large scale for the creation of transgenic organisms through insertion of genetic material into embryos, intracytoplasmic sperm injection, gene therapy and other related areas. While some of these processes have been automated, many, such as injection of material into suspended cells, i.e. embryos, are carried out manually by highly skilled workers using microscopes and manually controlled micro-robots. Although highly skilled, the success rate achieved by these workers is relatively low, thought to be due to the significant challenges to control insertion speeds and forces applied to biological cells, and variability of human manual control. Increases in throughput to meet current demand for these processes requires higher speeds for mechanical cell injection, electroporation and cell handling. Hence there exists a need to examine the effect of injector speeds, forces imparted on cell walls, injector retraction speeds, etc. on cell survival rates to be able to reliably design automated cell handling systems, controllers and automated systems to carry out automated injection.

生物学领域的最新进展提高了人们对可靠技术的兴趣和需求，这些技术可以通过将遗传物质插入胚胎、胞质内精子注射、基因治疗和其他相关领域来大规模地进行，以产生转基因生物体。虽然这些过程中的一些已经自动化，但许多过程，例如将材料注入悬浮细胞，即胚胎，是由高度熟练的工人使用显微镜和手动控制的微型机器人手动进行的。尽管这些工人技术高超，但成功率相对较低，这被认为是由于控制插入速度和施加到生物细胞的力的重大挑战以及人类手动控制的可变性。为满足这些过程的当前需求而增加的生产量需要更高的机械细胞注射、电穿孔和细胞处理速度。 因此，需要检查注射器速度、施加在细胞壁上的力、注射器缩回速度等对细胞存活率的影响，以能够可靠地设计自动化细胞处理系统、控制器和自动化系统来进行自动化注射。