High-speed centrifuge for multi-user molecular core facility

多用户分子核心设施高速离心机

• 批准号: 375628-2009
• 负责人: Godt, Dorothea
• 金额: $ 2.67万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412191
• 关键词: speed; centrifuge; multi; user; molecular

We propose to purchase a high volume high-speed centrifuge (Sorvall RC-6 Plus Superspeed) for the multi-user molecular core facility in our Department of Cell and Systems Biology. Molecular analysis aimed towards an understanding of gene and protein function in cell and tissue development is a major activity in our seven laboratories. Isolation of biological macromolecules (DNA, RNA and proteins) is an essential part of our research. The proposed centrifuge is required to harvest bacteria, to isolate DNA, RNA, and proteins at medium to large scale, and for fractionation of eukaryotic cells. The proposed instrument is urgently needed to replace the two centrifuges in our molecular core facility that both broke down (one in 2007, the other one in 2008). They were heavily used over the past 20 years by more than 15 research groups. Both centrifuges had to be decommissioned. This means that more than 15 research groups department, which do molecular biology, have currently no access to a centrifuge in our research building (Ramsay Wright Building) and have to take their samples to another building on campus for centrifugation. This causes substantial delays and impairs research quality, which is unacceptable. The proposed centrifuge will be in high demand as the seven laboratories combined train 20 graduate students, 5 postdocs, 6 technicians, and 19 undergraduate students. The centrifuge will also be available to other members of our Department. This instrument is critical to sustain high research productivity and quality. Research supported by the instrument will contribute to a better understanding of the molecular mechanisms of cell and tissue development, and associated abnormalities that result in diseases, including cancer, sterility, blindness, and deafness.

我们建议为我们的细胞和系统生物学系的多用户分子核心设施购买一台大容量高速离心机（Sorvall RC-6 Plus Superspeed）。旨在了解细胞和组织发育中基因和蛋白质功能的分子分析是我们七个实验室的主要活动。生物大分子（DNA，RNA和蛋白质）的分离是我们研究的重要组成部分。所提出的离心机需要收获细菌，分离DNA，RNA和蛋白质在中等到大规模，并用于真核细胞的分级分离。我们迫切需要拟议中的仪器来取代我们分子核心设施中的两台离心机，这两台离心机都发生了故障（一台在2007年，另一台在2008年）。在过去的20年里，超过15个研究小组大量使用它们。两台离心机都必须退役。这意味着，超过15个研究小组部门，做分子生物学，目前没有进入我们的研究大楼（拉姆齐赖特大楼）的离心机，并已采取他们的样品在校园内的另一个建筑离心。这造成了严重的延误，损害了研究质量，这是不可接受的。拟议的离心机将在高需求的七个实验室联合培训20名研究生，5名博士后，6名技术人员和19名本科生。该离心机也将提供给我们部的其他成员。这一工具对于维持高研究生产力和质量至关重要。该仪器支持的研究将有助于更好地了解细胞和组织发育的分子机制，以及导致癌症、不育、失明和耳聋等疾病的相关异常。