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Shared instruments (luminometer and real-time thermocycler) for gene expression analysis

用于基因表达分析的共享仪器（光度计和实时热循环仪）

基本信息

批准号：
376152-2009
负责人：
Ananvoranich, Sirinart
金额：
$ 3.71万
依托单位：
University of Windsor
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2008
资助国家：
加拿大
起止时间：
2008-01-01 至 2009-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412315
关键词：
Shared instruments luminometer real time

项目摘要

Gene expression analysis plays a pivotal role in cell and biochemical researches. While classical detection methods are still accepted, modern and highly sensitive techniques have been widely and overwhelmingly demanded by researchers and scientific journals. To maintain our competitiveness in research and training, we are seeking a financial support for the acquisition of a highly sensitive luminometer (20/20n Tube Luminometer by Turner Biosystems) and a real time thermocycler (StepOnePlusTM by Applied Biosystems). These state-of-the-art instruments are essential and will be heavily used by six research groups in the Department of Chemistry and Biochemistry (University of Windsor). The requested luminometer will be extensively used for dual luciferase reporter assays to determine the functions of promoters and regulatory elements; while the real time thermocycler will be extensively used for quantitative analyses, including genotyping and gene expression profiling. Currently there is no equivalent system available in the Department. The acquisition of these instruments will have tremendous and positive impact on the quality and productivity of the on-going and newly developed research programs, as well as on the training of highly qualified personnel.

基因表达分析在细胞和生物化学研究中起着至关重要的作用。虽然经典的检测方法仍然被接受，但研究人员和科学期刊已经广泛和压倒性地要求现代和高灵敏度的技术。为了保持我们在研究和培训方面的竞争力，我们正在寻求财政支持，以购买高灵敏度的光度计（Turner Biosystems的20/20 n管光度计）和真实的时间热循环仪（Applied Biosystems的StepOnePlusTM）。这些国家的最先进的仪器是必不可少的，并将大量使用的化学和生物化学系（温莎大学）的六个研究小组。所要求的光度计将广泛用于双荧光素酶报告基因检测，以确定启动子和调控元件的功能;而真实的时间热循环仪将广泛用于定量分析，包括基因分型和基因表达谱分析。目前，该部没有类似的系统。收购这些仪器将对正在进行的和新开发的研究计划的质量和生产力以及高素质人才的培训产生巨大的积极影响。