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Role of translational repression in Toxoplasma gondii's life cycle

翻译抑制在弓形虫生命周期中的作用

基本信息

批准号：
RGPIN-2018-04839
负责人：
Ananvoranich, Sirinart
金额：
$ 2.62万
依托单位：
University of Windsor
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2022
资助国家：
加拿大
起止时间：
2022-01-01 至 2023-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748438
关键词：
Role translational repression Toxoplasma gondii

项目摘要

Role of translational repression in Toxoplasma gondii's life cycleToxoplasma gondii is an intracellular parasite whose main hosts are rodents and cats, but it can also infect humans. It can affect its hosts' behavior and decision making, and causes toxoplasmosis. This parasitic infection is harmful to fetuses, and could be lethal to those with weak immune systems.This research program focuses on seeking a better understanding of the molecular mechanisms of T. gondii parasitism. More specifically, the research aims to identify molecular events critical to T. gondii's ability to establish an acute and latent infection by paying close attention to the interactions between mRNA and its interacting proteins in the formation of functional complexes necessary for protein synthesis. We hypothesize that translational repression in T. gondii plays a critical role in enabling it to establish infection and evade the host's protective responses. The research will develop and use novel and highly sensitive methods to monitor protein synthesis during different phases of the parasites in the human host cell. Our long-term objectives are to reveal the fundamental processes of the infection and pathogenesis, and to obtain mechanistic models that explain fundamental molecular processes of global and mRNA-specific translational repression in T. gondii. Short-term objectives: Using molecular/cell biology and biochemistry approaches, we will:1. Determine the mechanism of translational repression occurring in T. gondii that allows it to complete its life cycle in human host cells. 2. Evaluate the significance and function of an RNA-binding protein, named TgHoDI, in translational repression.3. Assess the mechanism by which a specific set of mRNAs are repressed during the dormant phase of the parasite infection in human host cells.Anticipated significance: The proposed research will advance our understanding of the parasite at the cellular and biochemical levels. The results will provide scientifi

翻译抑制在弓形虫生命周期中的作用弓形虫是一种细胞内寄生虫，主要宿主是啮齿类动物和猫，但它也可以感染人类。它可以影响宿主的行为和决策，并导致弓形虫病。这种寄生虫感染对胎儿是有害的，对免疫系统较弱的人可能是致命的。弓形虫寄生更具体地说，这项研究旨在确定T。弓形虫通过密切关注mRNA及其相互作用蛋白质在蛋白质合成所需的功能复合物形成过程中的相互作用，建立急性和潜伏感染的能力。我们推测T.弓形虫在建立感染和逃避宿主保护性反应方面起着关键作用。该研究将开发和使用新的高灵敏度方法来监测寄生虫在人类宿主细胞中不同阶段的蛋白质合成。我们的长期目标是揭示感染和发病机制的基本过程，并获得机制模型，解释在T.刚地。短期目标：利用分子/细胞生物学和生物化学方法，我们将：1。确定T.这使得它能够在人类宿主细胞中完成其生命周期。2.评估RNA结合蛋白TgHoDI在翻译抑制中的意义和功能.评估在寄生虫感染人类宿主细胞的休眠期期间一组特定mRNA被抑制的机制。预期意义：拟议的研究将促进我们在细胞和生化水平上对寄生虫的理解。研究结果将为科学家提供