Acquisition of a highly efficient lysis and ultracentrifugation system

本申请的目的是获得一种专门的细胞破碎和超离心系统，用于蛋白质提取物的制备、细胞器的分离和分子复合物的精细分离。 细胞裂解是细胞分级分离的第一步，因此，打开了无数生物学研究的大门。 所有的细胞都有一个质膜，一个蛋白质-脂质双层，形成一个屏障，将细胞内容物与细胞外环境隔开。 对于细菌（特别是革兰氏阳性菌）、真菌和植物细胞，质膜也被细胞壁包围，细胞壁是由多糖和蛋白质组成的复合结构。 细胞壁既坚固又有弹性，从而使细胞免受生物体遇到的恶劣环境的影响。 由于我们的研究使用细菌、酵母和植物细胞作为模型系统，因此我们必须能够获得高效的多样品珠细胞破碎机，以便从耐药生物样品中有效提取DNA/RNA、蛋白质和细胞器内容物。

The goal of this application is to acquire a specialized cell disrupting and ultracentrifugation system for the preparation of protein extracts, isolation of organelles, and fine separation of molecular complexes. Cell lysis is the first step in cell fractionation, and as such, opens the door to a myriad of biological studies. All cells have a plasma membrane, a protein-lipid bilayer that forms a barrier separating cell contents from the extracellular environment. For bacteria (especially Gram-positive), fungi, and plant cells, the plasma membrane is also surrounded by a cell wall, which is a composite structure that consists of polysaccharides and proteins. The cell wall is both firm-to-rupture and elastic, thereby fortifying the cell from hostile environments encountered by the organism. Because our studies use bacteria, yeasts, and plant cells as model systems, it is crucial that we can have access to a highly efficient multi-sample bead cell disrupter in order to efficiently extract DNA/RNA, protein, and organelle content from resistant biological samples.