Acquisition of a highly efficient lysis and ultracentrifugation system

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The goal of this application is to acquire a specialized cell disrupting and ultracentrifugation system for the preparation of protein extracts, isolation of organelles, and fine separation of molecular complexes. Cell lysis is the first step in cell fractionation, and as such, opens the door to a myriad of biological studies. All cells have a plasma membrane, a protein-lipid bilayer that forms a barrier separating cell contents from the extracellular environment. For bacteria (especially Gram-positive), fungi, and plant cells, the plasma membrane is also surrounded by a cell wall, which is a composite structure that consists of polysaccharides and proteins. The cell wall is both firm-to-rupture and elastic, thereby fortifying the cell from hostile environments encountered by the organism. Because our studies use bacteria, yeasts, and plant cells as model systems, it is crucial that we can have access to a highly efficient multi-sample bead cell disrupter in order to efficiently extract DNA/RNA, protein, and organelle content from resistant biological samples.