Comparative genomics of fungi

真菌比较基因组学

• 批准号: 105618-2007
• 负责人: Hsiang, Tom
• 金额: $ 1.05万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=421744
• 关键词: Comparative; genomics; fungi

Fungi are of primary importance in organic matter degradation, as well as having major economic significance as agents of diseases of plants and animals, and in industrial fermentation. Fungi generally have the smallest genome sizes among higher eukaryotes. Consequently, there are more genomes of fungi sequenced than those of animals or plants, with over 45 fungal genomes publicly available to date. The fungal genomes sequenced and the advanced annotation provide a data-rich resource with easy accessibility. I propose to examine the biology and evolution of fungi and some other promising microbial eukaryotes such as slime molds (myxomycota) using genetic data from online sources supplemented by data obtained experimentally in my research program. The purpose of this work is to investigate the evolutionary relationships of fungi, myxomycota and other microbial eukaryotes by searching for new gene sequences with or without annotation from sequenced genomes which may be useful in phylogenetic analyses at various levels, and supplementing the existing sequences and data with wet lab work. These objectives are part of a long term program investigating the evolution and biology of fungi, using the new tools of comparative genomics combined with basic sequencing and sequence analysis techniques. Their eukaryotic nature and small genome sizes make fungi particularly valuable in comparative genomics, and, with the exponentially increasing amounts of sequence data, it is now possible to access and analyse these data to bring forth important discoveries of biological significance.

真菌在有机物降解中具有首要重要性，并且作为植物和动物疾病的媒介以及在工业发酵中具有重要的经济意义。真菌通常在高等真核生物中具有最小的基因组大小。 因此，测序的真菌基因组比动物或植物基因组要多，迄今为止已公开的真菌基因组超过45个。真菌基因组测序和先进的注释提供了一个数据丰富的资源，易于访问。我建议研究真菌和其他一些有前途的微生物真核生物的生物学和进化，如黏菌（粘菌门），使用在线来源的遗传数据，并辅以我的研究计划中实验获得的数据。本工作的目的是通过从已测序的基因组中寻找新的基因序列（有或没有注释）来研究真菌、粘菌门和其他微生物真核生物的进化关系，这可能在不同水平上的系统发育分析中有用，并通过湿实验室工作补充现有的序列和数据。这些目标是一个长期计划的一部分，调查真菌的进化和生物学，使用比较基因组学的新工具，结合基本的测序和序列分析技术。它们的真核生物性质和小的基因组大小使真菌在比较基因组学中特别有价值，并且随着序列数据的数量呈指数级增长，现在可以访问和分析这些数据，以带来具有生物学意义的重要发现。