Unraveling The Mechanism Of Cryptic Sexual Cycle Development In Amoebae Using Comparative Genomics And Cytology
利用比较基因组学和细胞学揭示阿米巴原虫隐性性周期发育的机制
基本信息
- 批准号:10047109
- 负责人:
- 金额:$ 39.02万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-18 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcanthamoebaAddressAmoeba genusAnimalsBehaviorBehavioralBiological ModelsBiological ProcessBrainCell Differentiation processCell NucleusCell physiologyCommunicable DiseasesComparative StudyComplementComplexCystCytokinesisCytological TechniquesCytologyDataData AnalysesDevelopmentDiploidyDiseaseEntamoeba histolyticaEnvironmentEukaryotaEvolutionEye InfectionsGenesGeneticGenetic RecombinationGenetic VariationGenomeHaploidyHealthHumanInfectious Skin DiseasesInheritance PatternsKnowledgeLaboratoriesLife Cycle StagesLightMeiosisMethodsMicrobeMolecularMutationNuclear FusionPathogenesisPathogenicityPathway interactionsPeriodicityPharmaceutical PreparationsPlayPloidiesPolyploidyPublishingReproductionResearchRoleSexual DevelopmentSexualitySignal TransductionSystemSystems DevelopmentTaxonTestingVariantVirulenceWorkasexualbasecell motilitycombatcomparative genomicsexperimental studyfitnessgenetic manipulationgenomic datahuman pathogeninsightmicrobialnew therapeutic targetpathogenplant fungipressurereproductivesextranscriptomics
项目摘要
PROJECT SUMMARY/ABSTRACT
Unraveling The Mechanism Of Cryptic Sexual Cycle Development In Amoebae
Using Comparative Genomics And Cytology
The proposed research aims to unravel the cytological and genetic evidence underlying
the cryptic sexual life cycle of two amoeboids: Cochliopodium and Acanthamoeba. Sexual
reproduction is highly beneficial in eukaryotes by enabling them to increase genetic
diversity, fitness and virulence as in pathogenic eukaryotes. However, sexuality in most
microbial eukaryotes is poorly understood due to their diverse and complex life cycle.
Unlike most other amoebae that are assumed to engage in sex during the dormant, cyst
stage (a challenge for experimental study); the target taxon, Cochliopodium, undergoes
nuclear fusion in the actively growing vegetative stage making it an ideal candidate for
investigative work involving live experimentation. Our findings from previous work
with Cochliopodium have enabled us to make a comparative study with the unknown
aspects of the life cycle of a close relative opportunistic human pathogen, Acanthamoeba.
This amoeba causes severe infection of the eye, brain and skin infections. Acanthamoeba
is considered to reproduce asexually but recent genomic data indicated that it may
engage in cryptic sex. However, there is no corroborative evidence from behavioral or
cytological data to support this claim. In this proposal, we use the knowledge gained
from Cochliopodium to study the life cycle of Acanthamoeba with a similar approach.
The proposed work will test three hypotheses related to sexual development in
Cochliopodium and Acanthamoeba. These hypotheses are based from our previous
published, on-going research and new discoveries. We will use a combination of
methods including comparative genomics, transcriptomics, genetic manipulations and
optimized cytological techniques to tackle the proposed hypotheses.
Addressing the proposed hypotheses will enable the following new discoveries: (a)
uncover cytological signatures of the stages of sexual development and (b) shed light on
the molecular mechanisms of the cryptic sexual life cycle in Cochliopodium and
Acanthamoeba; (c) patterns of inheritance in Cochliopodium; (d) setting a groundwork for
understanding the evolution of sexuality in microbial eukaryotes with cryptic life cycles;
(e). exploration of targets that might be used to combat eukaryotic pathogens.
项目总结/摘要
揭开阿米巴性周期发育的神秘机制
利用比较基因组学和细胞学
这项拟议中的研究旨在揭示潜在的细胞学和遗传学证据。
两种变形虫的神秘性生活周期:旋足虫和阿米巴。性
真核生物的繁殖是非常有益的,
多样性、适应性和毒力。然而,在大多数情况下,
微生物真核生物由于其多样性和复杂的生命周期而知之甚少。
不像大多数其他变形虫,被认为是从事性在休眠期,囊肿
阶段(对实验研究的挑战);目标分类群,旋足虫,经历
在活跃生长的营养阶段进行核聚变,使其成为
涉及活体实验的调查工作。我们从以前的工作中发现
使我们能够与未知的进行比较研究
一种近亲机会性人类病原体阿米巴的生命周期方面。
这种变形虫会导致眼睛、大脑和皮肤的严重感染。棘阿米巴
被认为是无性繁殖的,但最近的基因组数据表明,
进行神秘的性爱然而,没有确凿的证据,从行为或
细胞学数据来支持这一说法。在本提案中,我们使用所获得的知识
用类似的方法研究阿米巴的生活史。
这项拟议中的工作将测试与性发育有关的三个假设,
旋足虫和阿米巴原虫。这些假设是基于我们以前的
已发表的、正在进行的研究和新发现。我们将结合使用
方法包括比较基因组学、转录组学、遗传操作和
优化细胞学技术来解决提出的假设。
解决所提出的假设将使以下新的发现:(a)
揭示性发育阶段的细胞学特征和(B)阐明
旋柄藻隐性性生活史的分子机制,
(c)旋足虫属的遗传模式;(d)为以下方面奠定基础:
了解具有神秘生命周期的微生物真核生物的性进化;
(e).探索可能用于对抗真核病原体的靶点。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Omics of an Enigmatic Marine Amoeba Uncovers Unprecedented Gene Trafficking from Giant Viruses and Provides Insights into Its Complex Life Cycle.
- DOI:10.3390/microbiolres14020047
- 发表时间:2023-06
- 期刊:
- 影响因子:1.5
- 作者:Tekle YI;Tran H;Wang F;Singla M;Udu I
- 通讯作者:Udu I
Phylogenomics of Thecamoebida (Discosea, Amoebozoa) with the Description of Stratorugosa tubuloviscum gen. nov. sp. nov., a Freshwater Amoeba with a Perinuclear MTOC.
Thecamoebida(Discosea、Amoebozoa)的系统发育学及 Stratorugosa tubeluviscum gen 的描述。
- DOI:10.1016/j.protis.2018.09.002
- 发表时间:2019
- 期刊:
- 影响因子:2.5
- 作者:Melton3rd,JamesT;Wood,FionaC;Branch,Jordan;Singla,Mandakini;Tekle,YonasI
- 通讯作者:Tekle,YonasI
New insights on the evolutionary relationships between the major lineages of Amoebozoa.
- DOI:10.1038/s41598-022-15372-7
- 发表时间:2022-07-01
- 期刊:
- 影响因子:4.6
- 作者:Tekle, Yonas, I;Wang, Fang;Wood, Fiona C.;Anderson, O. Roger;Smirnov, Alexey
- 通讯作者:Smirnov, Alexey
Amoebozoans Are Secretly but Ancestrally Sexual: Evidence for Sex Genes and Potential Novel Crossover Pathways in Diverse Groups of Amoebae.
变形虫是秘密但祖先有性的:不同变形虫群体中性基因和潜在新交叉途径的证据。
- DOI:10.1093/gbe/evx002
- 发表时间:2017
- 期刊:
- 影响因子:3.3
- 作者:Tekle,YonasI;Wood,FionaC;Katz,LauraA;Cerón-Romero,MarioA;Gorfu,LydiaA
- 通讯作者:Gorfu,LydiaA
Differential gene expression analysis and cytological evidence reveal a sexual stage of an amoeba with multiparental cellular and nuclear fusion.
- DOI:10.1371/journal.pone.0235725
- 发表时间:2020
- 期刊:
- 影响因子:3.7
- 作者:Tekle YI;Wang F;Heidari A;Stewart AJ
- 通讯作者:Stewart AJ
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Yonas Tekle其他文献
Yonas Tekle的其他文献
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{{ truncateString('Yonas Tekle', 18)}}的其他基金
Unraveling the mechanism of cryptic sexual cycle in Amoeba, Cochliopodium, using comparative transcriptome and immunocytochemistry
利用比较转录组和免疫细胞化学揭示阿米巴、Cochliopodium 神秘性周期的机制
- 批准号:
8957502 - 财政年份:2015
- 资助金额:
$ 39.02万 - 项目类别:
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