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Unraveling The Mechanism Of Cryptic Sexual Cycle Development In Amoebae Using Comparative Genomics And Cytology

利用比较基因组学和细胞学揭示阿米巴原虫隐性性周期发育的机制

基本信息

批准号：
10047109
负责人：
Yonas Tekle
金额：
$ 39.02万
依托单位：
SPELMAN COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-18 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10047109
关键词：
Acanthamoeba Address Amoeba genus Animals Behavior Behavioral Biological Models Biological Process Brain Cell Differentiation process Cell Nucleus Cell physiology Communicable Diseases Comparative Study Complement Complex Cyst Cytokinesis Cytological Techniques Cytology Data Data Analyses Development Diploidy Disease Entamoeba histolytica Environment Eukaryota Evolution Eye Infections Genes Genetic Genetic Recombination Genetic Variation Genome Haploidy Health Human Infectious Skin Diseases Inheritance Patterns Knowledge Laboratories Life Cycle Stages Light Meiosis Methods Microbe Molecular Mutation Nuclear Fusion Pathogenesis Pathogenicity Pathway interactions Periodicity Pharmaceutical Preparations Play Ploidies Polyploidy Publishing Reproduction Research Role Sexual Development Sexuality Signal Transduction System Systems Development Taxon Testing Variant Virulence Work asexual base cell motility combat comparative genomics experimental study fitness genetic manipulation genomic data human pathogen insight microbial new therapeutic target pathogen plant fungi pressure reproductive sex transcriptomics

项目摘要

PROJECT SUMMARY/ABSTRACT Unraveling The Mechanism Of Cryptic Sexual Cycle Development In Amoebae Using Comparative Genomics And Cytology The proposed research aims to unravel the cytological and genetic evidence underlying the cryptic sexual life cycle of two amoeboids: Cochliopodium and Acanthamoeba. Sexual reproduction is highly beneficial in eukaryotes by enabling them to increase genetic diversity, fitness and virulence as in pathogenic eukaryotes. However, sexuality in most microbial eukaryotes is poorly understood due to their diverse and complex life cycle. Unlike most other amoebae that are assumed to engage in sex during the dormant, cyst stage (a challenge for experimental study); the target taxon, Cochliopodium, undergoes nuclear fusion in the actively growing vegetative stage making it an ideal candidate for investigative work involving live experimentation. Our findings from previous work with Cochliopodium have enabled us to make a comparative study with the unknown aspects of the life cycle of a close relative opportunistic human pathogen, Acanthamoeba. This amoeba causes severe infection of the eye, brain and skin infections. Acanthamoeba is considered to reproduce asexually but recent genomic data indicated that it may engage in cryptic sex. However, there is no corroborative evidence from behavioral or cytological data to support this claim. In this proposal, we use the knowledge gained from Cochliopodium to study the life cycle of Acanthamoeba with a similar approach. The proposed work will test three hypotheses related to sexual development in Cochliopodium and Acanthamoeba. These hypotheses are based from our previous published, on-going research and new discoveries. We will use a combination of methods including comparative genomics, transcriptomics, genetic manipulations and optimized cytological techniques to tackle the proposed hypotheses. Addressing the proposed hypotheses will enable the following new discoveries: (a) uncover cytological signatures of the stages of sexual development and (b) shed light on the molecular mechanisms of the cryptic sexual life cycle in Cochliopodium and Acanthamoeba; (c) patterns of inheritance in Cochliopodium; (d) setting a groundwork for understanding the evolution of sexuality in microbial eukaryotes with cryptic life cycles; (e). exploration of targets that might be used to combat eukaryotic pathogens.

项目总结/摘要揭开阿米巴性周期发育的神秘机制利用比较基因组学和细胞学这项拟议中的研究旨在揭示潜在的细胞学和遗传学证据。两种变形虫的神秘性生活周期：旋足虫和阿米巴。性真核生物的繁殖是非常有益的，多样性、适应性和毒力。然而，在大多数情况下，微生物真核生物由于其多样性和复杂的生命周期而知之甚少。不像大多数其他变形虫，被认为是从事性在休眠期，囊肿阶段（对实验研究的挑战）;目标分类群，旋足虫，经历在活跃生长的营养阶段进行核聚变，使其成为涉及活体实验的调查工作。我们从以前的工作中发现使我们能够与未知的进行比较研究一种近亲机会性人类病原体阿米巴的生命周期方面。这种变形虫会导致眼睛、大脑和皮肤的严重感染。棘阿米巴被认为是无性繁殖的，但最近的基因组数据表明，进行神秘的性爱然而，没有确凿的证据，从行为或细胞学数据来支持这一说法。在本提案中，我们使用所获得的知识用类似的方法研究阿米巴的生活史。这项拟议中的工作将测试与性发育有关的三个假设，旋足虫和阿米巴原虫。这些假设是基于我们以前的已发表的、正在进行的研究和新发现。我们将结合使用方法包括比较基因组学、转录组学、遗传操作和优化细胞学技术来解决提出的假设。解决所提出的假设将使以下新的发现：（a）揭示性发育阶段的细胞学特征和（B）阐明旋柄藻隐性性生活史的分子机制，（c）旋足虫属的遗传模式;（d）为以下方面奠定基础：了解具有神秘生命周期的微生物真核生物的性进化; （e）.探索可能用于对抗真核病原体的靶点。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Omics of an Enigmatic Marine Amoeba Uncovers Unprecedented Gene Trafficking from Giant Viruses and Provides Insights into Its Complex Life Cycle.

DOI：
10.3390/microbiolres14020047
发表时间：
2023-06
期刊：
Microbiology research
影响因子：
1.5
作者：
Tekle YI;Tran H;Wang F;Singla M;Udu I
通讯作者：
Udu I

Phylogenomics of Thecamoebida (Discosea, Amoebozoa) with the Description of Stratorugosa tubuloviscum gen. nov. sp. nov., a Freshwater Amoeba with a Perinuclear MTOC.

Thecamoebida（Discosea、Amoebozoa）的系统发育学及 Stratorugosa tubeluviscum gen 的描述。

DOI：
10.1016/j.protis.2018.09.002
发表时间：
2019
期刊：
Protist
影响因子：
2.5
作者：
Melton3rd,JamesT;Wood,FionaC;Branch,Jordan;Singla,Mandakini;Tekle,YonasI
通讯作者：
Tekle,YonasI

New insights on the evolutionary relationships between the major lineages of Amoebozoa.

DOI：
10.1038/s41598-022-15372-7
发表时间：
2022-07-01
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Tekle, Yonas, I;Wang, Fang;Wood, Fiona C.;Anderson, O. Roger;Smirnov, Alexey
通讯作者：
Smirnov, Alexey

Amoebozoans Are Secretly but Ancestrally Sexual: Evidence for Sex Genes and Potential Novel Crossover Pathways in Diverse Groups of Amoebae.

变形虫是秘密但祖先有性的：不同变形虫群体中性基因和潜在新交叉途径的证据。