Transcription factor binding Determinates

转录因子结合决定因素

• 批准号: nhmrc : GNT1148380
• 负责人: Dr Luke Isbel
• 金额: $ 40.88万
• 依托单位国家: 澳大利亚
• 项目类别: Early Career Fellowships
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 无数据
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/transcription-factor-binding-determinates/1348875
• 关键词: Transcription; factor; binding; Determinates

Transcription factors (TFs) read cis-regulatory information encoded in the genome. However, they must compete with structural chromatin proteins such as nucleosomes for access to DNA motifs. It is unclear what features discriminate used from unused binding sites with similar motifs, in vivo. To provide insight and general principles of TF biology, I will express variants of a model TF in mammalian cells and test the contribution that specific molecular properties have on genomic binding.

转录因子（TF）读取基因组中编码的顺式调控信息。然而，它们必须与结构染色质蛋白如核小体竞争进入DNA基序。目前还不清楚什么功能区分使用与未使用的结合位点具有相似的基序，在体内。为了提供TF生物学的见解和一般原则，我将在哺乳动物细胞中表达模型TF的变体，并测试特定分子特性对基因组结合的贡献。