Transcription factor binding Determinates

转录因子（TFs）读取基因组编码的顺式调控信息。然而，它们必须与结构染色质蛋白（如核小体）竞争以获得DNA基序。目前尚不清楚在体内，哪些特征区分了使用的与未使用的具有相似基序的结合位点。为了深入了解TF生物学的一般原理，我将在哺乳动物细胞中表达模型TF的变体，并测试特定分子特性对基因组结合的贡献。

Transcription factors (TFs) read cis-regulatory information encoded in the genome. However, they must compete with structural chromatin proteins such as nucleosomes for access to DNA motifs. It is unclear what features discriminate used from unused binding sites with similar motifs, in vivo. To provide insight and general principles of TF biology, I will express variants of a model TF in mammalian cells and test the contribution that specific molecular properties have on genomic binding.