Transcription factor binding Determinates

转录因子结合决定因素

• 批准号: nhmrc : 1148380
• 金额: $ 27.82万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Early Career Fellowships
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/transcription-factor-binding-determinates/1321003
• 关键词: Transcription; factor; binding; Determinates

Transcription factors (TFs) read cis-regulatory information encoded in the genome. However, they must compete with structural chromatin proteins such as nucleosomes for access to DNA motifs. It is unclear what features discriminate used from unused binding sites with similar motifs, in vivo. To provide insight and general principles of TF biology, I will express variants of a model TF in mammalian cells and test the contribution that specific molecular properties have on genomic binding.

转录因子(TF)读取基因组中编码的顺式调控信息。然而，它们必须与核小体等结构染色质蛋白质竞争才能获得DNA基序。目前还不清楚在活体中，使用了哪些功能来区别于具有类似模体的未使用的结合位点。为了提供对转铁蛋白生物学的洞察力和一般原理，我将在哺乳动物细胞中表达模型转铁蛋白的变体，并测试特定的分子属性对基因组结合的贡献。