Diluters or extenders for mammalian sperm processing and preservation

用于哺乳动物精子处理和保存的稀释剂或增量剂

• 批准号: 387274-2009
• 负责人: Manjunath, Puttaswamy
• 金额: $ 8.67万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=442804
• 关键词: Diluters; extenders; mammalian; sperm; processing

The cryopreservation of bull semen represents one of the most important achievements in dairy farming after the introduction of artificial insemination. These two approaches have enabled the worldwide distribution and use of desired genetic lines at a reasonable cost. Canada represents <3% of the global economy but provides >20% of world bovine semen exports (~70 million dollars/annum). Egg yolk (EY) and milk are the most used protective agents to conserve sperm. EY and milk, being products of animal origin, represent a potential risk of contamination of semen. Thus, there is an urgent need of developing novel media free of products from animal origin to conserve semen. However, it is difficult to find replacement component for EY or milk because the mechanisms involved in sperm protection by EY and milk remain unclear. Our work has provided new insight into the mechanism of sperm protection by EY. We showed that seminal vesicles of all ungulates studied to

公牛精液的冷冻保存是继人工授精之后奶牛养殖业最重要的成就之一。这两种方法使得能够以合理的成本在世界范围内分配和使用所需的遗传品系。加拿大占<3% of the global economy but provides >世界牛精液出口量的20%（每年约7000万美元）。蛋黄（EY）和牛奶是保存精子最常用的保护剂。EY和牛奶是动物源性产品，存在精液污染的潜在风险。因此，迫切需要开发不含动物源性产品的新型培养基来保存精液。然而，很难找到替代EY或牛奶的成分，因为EY和牛奶保护精子的机制尚不清楚。我们的工作为EY的精子保护机制提供了新的见解。我们发现，所有有蹄类动物的精囊研究，