Mechanism of mammalian sperm protection by extender components and development of novel extenders free of products of animal origin

补充剂成分保护哺乳动物精子的机制以及不含动物源产品的新型补充剂的开发

• 批准号: RGPIN-2017-05777
• 负责人: Manjunath, Puttaswamy
• 金额: $ 2.04万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630999
• 关键词: Mechanism; mammalian; sperm; protection; extender

Canada exported more than 110 million dollars worth of bull semen to over 100 countries in 2015 (Agriculture Canada). This represents >20% of world export of bull semen. The most commonly used protective agents to conserve mammalian sperm contain 20% egg yolk (EY) in Tris-/citrate buffers or skimmed/whole milk and 6-7% glycerol. This basic constituent of sperm preservation media (also called diluters or extenders) has remained the same since the practice of artificial insemination was introduced some 70 years ago.

2015年，加拿大向100多个国家出口了价值超过1.1亿美元的公牛精液（加拿大农业部）。这相当于世界公牛精液出口量的20%。最常用的保存哺乳动物精子的保护剂含有20%的蛋黄（EY）在Tris-/柠檬酸缓冲液或脱脂/全脂牛奶和6-7%的甘油。精子保存介质（也称为稀释剂或扩展剂）的基本成分自70年前人工授精以来一直保持不变。