Quantitative Real time Thermal Cycler

实时定量热循环仪

• 批准号: 389585-2010
• 负责人: Bentzen, Paul
• 金额: $ 3.67万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=442959
• 关键词: Quantitative; Real; time; Thermal; Cycler

The requested quantitative real-time thermal cycler will allow selected DNA and RNA sequences to be simultaneously amplified and quantified. The instrument will be housed in a multi-user laboratory, the Marine Gene Probe Laboratory, where it will be put to a wide variety of uses. The two main uses, however, will be genotyping single nucleotide polymorphisms (SNPs) in a wide range of organisms, and quantifying the level of expression of particular genes. In combination, these two procedures will be used to study population structure, dispersal, evolutionary relationships and local adaptations in marine and aquatic organisms. This research will in turn be applied to fisheries management and conservation of fishes and aquatic invertebrates.

所要求的实时定量热循环仪将允许选定的DNA和RNA序列同时被扩增和定量。该仪器将被安置在一个多用户实验室--海洋基因探测实验室，在那里它将被用于广泛的各种用途。然而，这两个主要用途将是对广泛生物中的单核苷酸多态(SNPs)进行基因分型，以及量化特定基因的表达水平。这两个程序将被用于研究海洋和水生生物的种群结构、扩散、进化关系和局部适应。这项研究将反过来应用于鱼类和水生无脊椎动物的渔业管理和养护。